近零磁场下干扰磁响应关键基因对褐飞虱寿命的影响
贺静澜,张明,刘瑞莹,万贵钧,潘卫东,陈法军

Effects of the Interference of Key Magnetic Response Genes on the Longevity of Brown Planthopper (Nilaparvata lugens) Under Near-Zero Magnetic Field
HE JingLan,ZHANG Ming,LIU RuiYing,WAN GuiJun,PAN WeiDong,CHEN FaJun
表1 本试验所用引物及序列
Table 1 Primers and their sequences used in this study
引物用途 Use of primers 引物名称 Primer name 引物序列 Primer sequence (5′-3′)
cDNA克隆
cDNA cloning
dsNlCry1-F TAATACGACTCACTATAGGGTCAGACATGGGCTTCGATT
dsNlCry1-R TAATACGACTCACTATAGGGCATTTGGTAAGTTAGCGGTGGA
dsNlCry2-F TAATACGACTCACTATAGGGTGTCAGCATCAATAAGTGGAGG
dsNlCry2-R TAATACGACTCACTATAGGGGCACACCAAACTTGTCGTC
dsNlMagR-F TAATACGACTCACTATAGGGAGAAAGGAAAGTTTGACGAAG
dsNlMagR-R TAATACGACTCACTATAGGGAGCCCTAAATATTAACATCGT
dsGFP-F TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTTC
dsGFP-R TAATACGACTCACTATAGGGTGTTCTGCTGGTAGTGGTCG
实时荧光定量PCR
RT-qPCR
qNlCry1-F CAGACATGGGCTTCGATTTCA
qNlCry1-R ACCAGCACTTTCTCCGTCAAAT
qNlCry2-F CGCATACTCTCTACAGACTTGAT
qNlCry2-R CACCGTCTGGAATTTGCGATAC
qNlMagR-F CGTTTAATACCTTCAAGAGCAGCAC
qNlMagR-R CCCTACTTTCAAGCCGATAGCAT
qActin-F CTTCTAAACGCCAACCACTCC
qActin-R TCACCCGAAATCACTCACGA
q18S-F TGTCTGCTTAATTGCGATAACGAAC
q18S-R CCTCAAACTTCCATCGGCTTG