近零磁场下干扰磁响应关键基因对褐飞虱寿命的影响

贺静澜,张明,刘瑞莹,万贵钧,潘卫东,陈法军

Effects of the Interference of Key Magnetic Response Genes on the Longevity of Brown Planthopper (Nilaparvata lugens) Under Near-Zero Magnetic Field

HE JingLan,ZHANG Ming,LIU RuiYing,WAN GuiJun,PAN WeiDong,CHEN FaJun

表1 本试验所用引物及序列

Table 1 Primers and their sequences used in this study

引物用途 Use of primers 引物名称 Primer name 引物序列 Primer sequence (5′-3′) cDNA克隆 cDNA cloning dsNlCry1-F TAATACGACTCACTATAGGGTCAGACATGGGCTTCGATT dsNlCry1-R TAATACGACTCACTATAGGGCATTTGGTAAGTTAGCGGTGGA dsNlCry2-F TAATACGACTCACTATAGGGTGTCAGCATCAATAAGTGGAGG dsNlCry2-R TAATACGACTCACTATAGGGGCACACCAAACTTGTCGTC dsNlMagR-F TAATACGACTCACTATAGGGAGAAAGGAAAGTTTGACGAAG dsNlMagR-R TAATACGACTCACTATAGGGAGCCCTAAATATTAACATCGT dsGFP-F TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTTC dsGFP-R TAATACGACTCACTATAGGGTGTTCTGCTGGTAGTGGTCG 实时荧光定量PCR RT-qPCR qNlCry1-F CAGACATGGGCTTCGATTTCA qNlCry1-R ACCAGCACTTTCTCCGTCAAAT qNlCry2-F CGCATACTCTCTACAGACTTGAT qNlCry2-R CACCGTCTGGAATTTGCGATAC qNlMagR-F CGTTTAATACCTTCAAGAGCAGCAC qNlMagR-R CCCTACTTTCAAGCCGATAGCAT qActin-F CTTCTAAACGCCAACCACTCC qActin-R TCACCCGAAATCACTCACGA q18S-F TGTCTGCTTAATTGCGATAACGAAC q18S-R CCTCAAACTTCCATCGGCTTG