意大利蜜蜂幼虫肠道在球囊菌侵染前期的 差异表达microRNA及其调控网络

郭睿,杜宇,童新宇,熊翠玲,郑燕珍,徐国钧,王海朋,耿四海,周丁丁,郭意龙,吴素珍,陈大福

Differentially Expressed MicroRNAs and Their Regulation Networks in Apis mellifera ligustica Larval Gut During the Early Stage of Ascosphaera apis Infection

GUO Rui,DU Yu,TONG XinYu,XIONG CuiLing,ZHENG YanZhen,XU GuoJun,WANG HaiPeng,GENG SiHai,ZHOU DingDing,GUO YiLong,WU SuZhen,CHEN DaFu

表1 本研究使用的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence LOOP-miR-3793-x CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGCCAGG LOOP-ame-miR-6000a-5p CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATAGAGAC LOOP-novel-m0031-3p CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCCTGCTT LOOP-novel-m0034-5p CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATATCACA F-miR-3793-x ACACTCCAGCTGGGAGCGTGTTTTC F-ame-miR-6000a-5p ACACTCCAGCTGGGCAGCAGCAGCAG F-novel-m0031-3p GCATCCTCTTGAAT F-novel-m0034-5p CAGGTAACTACTGC R CTCAACTGGTGTCGTGGA U6-F GTTAGGCTTTGACGATTTCG U6-R GGCATTTCTCCACCAGGTA