牛LncRNA-133a对骨骼肌卫星细胞增殖分化的影响

李燕,陈明明,张俊星,张林林,李新,郭宏,丁向彬,刘新峰

Effects of Bovine LncRNA-133a on the Proliferation and Differentiation of Skeletal Muscle Satellite Cells

LI Yan,CHEN MingMing,ZHANG JunXing,ZHANG LinLin,LI Xin,GUO Hong,DING XiangBin,LIU XinFeng

表1 qRT-PCR引物信息

Table 1 The primer information of qRT-PCR

基因 ID Gene ID 引物序列(5′-3′) Primers sequence (5′-3′) 产物长度 Product length (bp) LncRNA-133a Forward GCATAGCCGGTGTCTGAGAG 108 Reverse CGGCCGCTTGTATATTGTCC MHC Forward GTGGAATCCGGAGGCAGAA 105 Reverse TTTTCGAAGGTAGGGAGCGG MyoG Forward GGCTGACAAATGCCAGACTATCC 140 Reverse TGGTCCCTTGCTTTATCTCCCT MyoD Forward GACGGCTCTCTCTGCAACTT 101 Reverse CGGCGCGGATCCAGGT GAPDH-1 Forward ACAGTCAAGGCAGAGAACGG 98 Reverse CCAGCATCACCCCACTTGAT GAPDH-2 Forward CCTGCCCGTTCGACAGATAG 153 Reverse ATGGCGACGATGTCCACTTT