基于簇毛麦No.1026转录组的SSR序列分析及其PCR标记开发

陈竟男,马晓兰,王振,李仕金,谢皓,叶兴国,林志珊

SSR Sequences and Development of PCR Markers Based on Transcriptome of Dasypyrum villosum No.1026

CHEN JingNan,MA XiaoLan,WANG Zhen,LI ShiJin,XIE Hao,YE XingGuo,LIN ZhiShan

图4 EST-SSR引物225、EST-PCR引物6VS-12对簇毛麦的扩增图谱 M：Marker 5000;1—16：曾经与簇毛麦Dv#4近距离种植的Dv#2衍生的部分植株,簇毛麦Dv#2和Dv#4为原始亲本

Fig. 4 Amplification patterns of D. villosum by EST-SSR primer 225 and EST-PCR primers 6VS-12 M: Marker 2000, 1-16: The offspring individuals of Dv#2 that had been planted closely with Dv#4, D. villosum Dv#2 and Dv#4 are originals, respectively