基于簇毛麦No.1026转录组的SSR序列分析及其PCR标记开发

陈竟男,马晓兰,王振,李仕金,谢皓,叶兴国,林志珊

SSR Sequences and Development of PCR Markers Based on Transcriptome of Dasypyrum villosum No.1026

CHEN JingNan,MA XiaoLan,WANG Zhen,LI ShiJin,XIE Hao,YE XingGuo,LIN ZhiShan

图2 8对EST-SSR引物对CS、1V—7V异染色体系和簇毛麦的扩增图谱 M：Marker 2000,左侧为引物名称,右侧箭头显示引物扩增的特异条带及片段大小。下同

Fig. 2 Amplification patterns of CS, 1V-7V alien chromosome lines and D. villosum by 8 pairs of EST-SSR primers M: Marker 2000, name of primer is indicated on the left side, each of the amplified band is indicated by an arrow on the right side with the fragment size. The same as below