基于簇毛麦No.1026转录组的SSR序列分析及其PCR标记开发

陈竟男,马晓兰,王振,李仕金,谢皓,叶兴国,林志珊

SSR Sequences and Development of PCR Markers Based on Transcriptome of Dasypyrum villosum No.1026

CHEN JingNan,MA XiaoLan,WANG Zhen,LI ShiJin,XIE Hao,YE XingGuo,LIN ZhiShan

图1 EST-SSR引物对CS与2份簇毛麦的扩增结果 M：Marker 2000的部分区段,条带对应于100、250和500 bp;1—65表示65对不同引物,其扩增产物显示小麦与簇毛麦或簇毛麦间的多态性

Fig. 1 Amplification patterns of CS and two D. villosum accessions by EST- SSR primers M: Parts of Marker 2000, the bands are corresponding to 100 bp, 250 bp and 500 bp in size, respectively. 1-65 indicate 65 pairs of EST-SSR primers, the amplified products showing the PCR polymorphic fragments between wheat and D. villosum or between two D. villosum accessions