5个基因在小尾寒羊和苏尼特羊性腺轴相关组织中表达分析
张壮彪,狄冉,刘秋月,胡文萍,王翔宇,田志龙,张效生,张金龙,储明星

Expression Analysis of Five Genes in the Gonadal Axis of Small Tail Han Sheep and Sunite Sheep
ZHANG ZhuangBiao,DI Ran,LIU QiuYue,HU WenPing,WANG XiangYu,TIAN ZhiLong,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing
表3 实时荧光定量PCR体系
Table 3 System of real time fluorescent quantitative PCR
试剂
Reagent
各试剂加入量
Volume of each reagent (μL)
荧光定量Ex Taq引物Ⅱ
SYBR Premix Ex Taq
10.0
上游引物(10 μmol·L-1
Upstream primer (10 μmol·L-1)
0.8
下游引物(10 μmol·L-1
Downstream primer (10 μmol·L-1)
0.8
cDNA 模板(500 ng·μL-1
cDNA template (500 ng·μL-1)
2.0
双蒸水 RNase-Free ddH2O 6.4