5个基因在小尾寒羊和苏尼特羊性腺轴相关组织中表达分析

张壮彪,狄冉,刘秋月,胡文萍,王翔宇,田志龙,张效生,张金龙,储明星

Expression Analysis of Five Genes in the Gonadal Axis of Small Tail Han Sheep and Sunite Sheep

ZHANG ZhuangBiao,DI Ran,LIU QiuYue,HU WenPing,WANG XiangYu,TIAN ZhiLong,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing

表2 反转录体系

Table 2 System of reverse transcription

试剂 Reagent 各试剂加入量 Volume of each reagent PrimeScript 反转录混合引物Ⅰ PrimeScript RT Enzyme Mix Ⅰ 1.0 μL Oligo dT引物 Oligo dT Primer 1.0 μL 随机引物 Random 6 mers 1.0 μL 5×PrimeScript荧光定量缓冲液 5×PrimeScript Buffer (for Real Time) 4.0 μL RNA 1.0 μg 双蒸水 RNase-Free ddH2O 12μL