5个基因在小尾寒羊和苏尼特羊性腺轴相关组织中表达分析
|
张壮彪,狄冉,刘秋月,胡文萍,王翔宇,田志龙,张效生,张金龙,储明星
|
Expression Analysis of Five Genes in the Gonadal Axis of Small Tail Han Sheep and Sunite Sheep
|
ZHANG ZhuangBiao,DI Ran,LIU QiuYue,HU WenPing,WANG XiangYu,TIAN ZhiLong,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing
|
|
表1 引物的序列、扩增片段大小及退火温度
|
Table 1 The sequences,amplified products and Tm(annealing temperature)of PCR for six primer pairs
|
|
基因名称 Name | 引物序列 Primer sequence | 片段大小 Length (bp) | 退火温度 Tm (℃) | BMP2 | F:5′-ATCACCTGAACTCCACGAA-3′ | 140 | 60 | R: 5′-TACCACCTTCTCATTCTCATC-3′ | BMP6 | F:5′- AGCAGACTACAACAGCAGCGA-3′ | 267 | 60 | R:5′- AGCACCGAGATGGCGTTCA-3′ | BMP7 | F:5′- AAAACAGCAGCAGCGACCAGAG-3′ | 123 | 68 | R:5′- CCTCACAGTAGTAGGCGGCATAGC-3′ | CAST | F:5′-TGGGGCCCAATGACGCCATCGATG - 3′ | 222 | 60 | R:5′- GGTGGAGCAGCACTTCTGATCACC - 3′ | CART | F:5′- AGCAGCCACCATCAGAGAAA - 3′ | 214 | 60 | R:5′- TCCAGAGCAGTATCCATGTCT - 3′ | β-actin | F:5′- CCAACCGTGAGAAGATGACC-3′ | 97 | 60 | R:5′- CCCGAGGCGTACAGGGACAG-3′ |
|
|
|