5个基因在小尾寒羊和苏尼特羊性腺轴相关组织中表达分析

张壮彪,狄冉,刘秋月,胡文萍,王翔宇,田志龙,张效生,张金龙,储明星

Expression Analysis of Five Genes in the Gonadal Axis of Small Tail Han Sheep and Sunite Sheep

ZHANG ZhuangBiao,DI Ran,LIU QiuYue,HU WenPing,WANG XiangYu,TIAN ZhiLong,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing

表1 引物的序列、扩增片段大小及退火温度

Table 1 The sequences,amplified products and Tm（annealing temperature）of PCR for six primer pairs

基因名称 Name 引物序列 Primer sequence 片段大小 Length (bp) 退火温度 Tm (℃) BMP2 F:5′-ATCACCTGAACTCCACGAA-3′ 140 60 R: 5′-TACCACCTTCTCATTCTCATC-3′ BMP6 F:5′- AGCAGACTACAACAGCAGCGA-3′ 267 60 R:5′- AGCACCGAGATGGCGTTCA-3′ BMP7 F:5′- AAAACAGCAGCAGCGACCAGAG-3′ 123 68 R:5′- CCTCACAGTAGTAGGCGGCATAGC-3′ CAST F:5′-TGGGGCCCAATGACGCCATCGATG - 3′ 222 60 R:5′- GGTGGAGCAGCACTTCTGATCACC - 3′ CART F:5′- AGCAGCCACCATCAGAGAAA - 3′ 214 60 R:5′- TCCAGAGCAGTATCCATGTCT - 3′ β-actin F:5′- CCAACCGTGAGAAGATGACC-3′ 97 60 R:5′- CCCGAGGCGTACAGGGACAG-3′