多主棒孢SdhB-H278R突变位点AS-real-time PCR 定量检测体系的建立

孙炳学,石延霞,朱发娣,谢学文,柴阿丽,李宝聚

Establishment of AS-real-time PCR for Quantitatively Detecting the H278R Allele in the SdhB Associated with Corynespora cassiicola in Cucumber

SUN BingXue,SHI YanXia,ZHU FaDI,XIE XueWen,CHAI ALi,LI BaoJu

表5 AS-real-time PCR验证

Table 5 Verification of AS-real-time PCR

预期值Expected percentage (%) 80 40 20 10 5 检测值Detected percentage (%) DNA 85.95±1.45 44.64±0.69 22.24±0.85 11.86±0.60 6.40±0.24 孢子Spore 116.45±4.83 48.39±6.15 26.38±4.59 11.86±0.98 6.83±1.43