多主棒孢SdhB-H278R突变位点AS-real-time PCR 定量检测体系的建立

孙炳学,石延霞,朱发娣,谢学文,柴阿丽,李宝聚

Establishment of AS-real-time PCR for Quantitatively Detecting the H278R Allele in the SdhB Associated with Corynespora cassiicola in Cucumber

SUN BingXue,SHI YanXia,ZHU FaDI,XIE XueWen,CHAI ALi,LI BaoJu

Table 3 The biological characteristics of resistance and sensitivity strains

菌株编号 Strain code 敏感性 Sensitivity 菌丝生长速率（11d） Mycelium growth rate (cm·d-1) 产孢量 Spore outputs (×105/mL) 病斑直径 Lesion diameter (cm) HG17031007-2 S 0.60±0.09f 0.92±1.38a 0.78±0.09efg HG17031010-1 S 0.61±0.09f 7.00±4.61e 0.65±0.26de HG14102524-4 S 0.61±0.01f 3.85±1.99bcd 0.84±0.16fg HG17031008-2 S 0.49±0.06de 0.33±0.52a 0.38±0.10ab HG17031006-2 R 0.49±0.04de 5.15±2.41de 0.70±0.26def HG17031008-4 R 0.45±0.05cd 4.25±2.67bcd 0.75±0.17efg HG17031006-5 R 0.30±0.03a 2.31±2.06abc 0.56±0.19cd HG17031008-1 R 0.37±0.02ab 1.33±1.87a 0.45±0.22abc HG17031008-3 R 0.56±0.08ef 7.46±2.70e 0.91±0.12g HG17031008-5 R 0.52±0.01de 4.82±2.93cd 0.54±0.05bcd HG17031006-1 R 0.37±0.02ab 1.85±1.28ab 0.80±0.09efg HG17031009-5 R 0.40±0.01bc 4.00±2.80bcd 0.33±0.07a