多主棒孢SdhB-H278R突变位点AS-real-time PCR 定量检测体系的建立
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孙炳学,石延霞,朱发娣,谢学文,柴阿丽,李宝聚
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Establishment of AS-real-time PCR for Quantitatively Detecting the H278R Allele in the SdhB Associated with Corynespora cassiicola in Cucumber
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SUN BingXue,SHI YanXia,ZHU FaDI,XIE XueWen,CHAI ALi,LI BaoJu
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表2 供试菌株敏感性测定
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Table 2 Sensitivity of tested strains
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序号
Number | 菌株编号
Strain code | 采集时间
Collection time | 采集地
Collection area | EC50
(μg·mL-1) | 毒力回归方程Toxicity regression equation (y=) | 相关系数
Coefficient
(R2) | 突变类型Mutation type | 敏感性
Sensitivity | | 1 | HG17031006-1 | 2017 | 北京Beijing | 9.261 | 1.2509x+3.7941 | 0.9995 | + | R | | 2 | HG17031006-2 | 2017 | 北京Beijing | 5.925 | 1.1082x+4.1659 | 0.9788 | + | R | | 3 | HG17031006-3 | 2017 | 北京Beijing | 0.482 | 0.4848x+5.1535 | 0.9899 | - | S | | 4 | HG17031006-4 | 2017 | 北京Beijing | 0.227 | 0.4565x+5.2941 | 0.9910 | - | S | | 5 | HG17031006-5 | 2017 | 北京Beijing | 5.395 | 0.6544x+4.4397 | 0.9276 | + | R | | 6 | HG17031007-1 | 2017 | 北京Beijing | 0.337 | 0.4846x+5.2290 | 0.9905 | - | S | | 7 | HG17031007-2 | 2017 | 北京Beijing | 0.057 | 1.6824x+7.0839 | 0.9777 | - | S | | 8 | HG17031007-3 | 2017 | 北京Beijing | 0.261 | 2.1962x+4.9202 | 0.9905 | - | S | | 9 | HG17031007-4 | 2017 | 北京Beijing | 0.383 | 0.4912x+5.2047 | 0.9331 | - | S | | 10 | HG17031007-5 | 2017 | 北京Beijing | 0.221 | 0.5069x+5.9325 | 0.9746 | - | S | | 11 | HG17031008-1 | 2017 | 北京Beijing | 7.585 | 0.9496x+4.1644 | 0.9998 | + | R | | 12 | HG17031008-2 | 2017 | 北京Beijing | 0.535 | 1.5481x+5.8387 | 0.9219 | - | S | | 13 | HG17031008-3 | 2017 | 北京Beijing | 6.089 | 1.0695x+4.1608 | 0.9998 | + | R | | 14 | HG17031008-4 | 2017 | 北京Beijing | 7.408 | 1.0990x+4.0752 | 0.9961 | + | R | | 15 | HG17031008-5 | 2017 | 北京Beijing | 7.309 | 1.3476x+3.8337 | 0.9967 | + | R | | 16 | HG17031009-1 | 2017 | 北京Beijing | 0.495 | 1.1440x+5.6638 | 0.9038 | - | S | | 17 | HG17031009-2 | 2017 | 北京Beijing | 0.469 | 1.4358x+6.3270 | 0.9929 | - | S | | 18 | HG17031009-3 | 2017 | 北京Beijing | 0.550 | 1.1084x+5.9661 | 0.9944 | - | S | | 19 | HG17031009-4 | 2017 | 北京Beijing | 0.484 | 0.5298x+5.1666 | 0.9791 | - | S | | 20 | HG17031009-5 | 2017 | 北京Beijing | 11.710 | 0.6667x+4.2859 | 0.9154 | + | R | | 21 | HG17031010-1 | 2017 | 北京Beijing | 0.216 | 0.5158x+5.3436 | 0.9991 | - | S | | 22 | HG17031010-2 | 2017 | 北京Beijing | 0.563 | 1.3191x+5.6942 | 0.9225 | - | S | | 23 | HG17031010-4 | 2017 | 北京Beijing | 0.296 | 0.6165x+5.3264 | 0.9897 | - | S | | 24 | HG14102524-4 | 2014 | 河北Hebei | 0.316 | 2.9056x+3.5592 | 0.9945 | - | S | | 25 | HG17031010-5 | 2017 | 北京Beijing | 0.274 | 1.0630x+5.7384 | 0.9129 | - | S | | 26 | HG14102430-1 | 2014 | 河北Hebei | 22.371 | 0.9039x+3.7312 | 0.9652 | SdhB-H278Y | R | | 27 | HG15050729-5 | 2015 | 辽宁Liaoning | 3.954 | 0.9434x+4.4106 | 0.9960 | SdhB-I280V | R | | 28 | HG14102415-1 | 2014 | 河北Hebei | 1.111 | 0.8091x+4.4362 | 0.9967 | SdhB-P199S | R | | 29 | HG15050701-1 | 2015 | 辽宁Liaoning | 19.526 | 0.7921x+4.0567 | 0.9967 | SdhC-P73S | R |
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