多主棒孢SdhB-H278R突变位点AS-real-time PCR 定量检测体系的建立

孙炳学,石延霞,朱发娣,谢学文,柴阿丽,李宝聚

Establishment of AS-real-time PCR for Quantitatively Detecting the H278R Allele in the SdhB Associated with Corynespora cassiicola in Cucumber

SUN BingXue,SHI YanXia,ZHU FaDI,XIE XueWen,CHAI ALi,LI BaoJu

表1 引物信息

Table 1 Primer information

引物名称 Primer name 序列 Sequence 引物长度 Length of primer (bp) 产物长度 Length of product (bp) Cc-SdhB-F CACTCTTCTTCGCCATCC 18 1422 Cc-SdhB-R CATCACACTCACGGTCAC 18 B-H278R-2F GTGAATACCGCCAGTCCAA 19 244 B-H278R-2R14 CAGTTGAGAATCGCGC 16 B-H278R-TY-F GACCTTTAGGCGAAGTTGC 19 246 B-H278R-TY-R CTGCTTGTAGAAGAGCGTCAT 21