源于广8 A杂交稻组合的稻瘟病菌无毒基因型分析

汪文娟,苏菁,杨健源,韦小燕,陈凯玲,陈珍,陈深,朱小源

Analysis of Magnaporthe oryzae Avirulent Genes in the Infected Hybrid Rice Combinations Derived from a Sterile Line of Guang 8 A

WANG WenJuan,SU Jing,YANG JianYuan,WEI XiaoYan,CHEN KaiLing,CHEN Zhen,CHEN Shen,ZHU XiaoYuan

表2 本研究中用于检测无毒基因的引物

Table 2 Primers used for the PCR detection of Avr genes in this study

引物 Primer 引物序列 Sequence (5′-3′) 预期片段大小 Expected fragment size (bp) 目的 Purpose Avr1-CO39 F TGCCGCATTTTGCTAACCG 994 检测Avr1-CO39启动子及CDS To detect Avr1-CO39 promoter and CDS Avr1-CO39 R GCGAATCCATAGACAAGGAC AvrPita F CAGGCATACATTGGAGAGCC 1549 检测AvrPita启动子及CDS To detect AvrPita promoter and CDS AvrPita R CCCTCCATTCCAACACTAAC AvrPii F GGTAGATATCCGCTGACTGG 839 检测AvrPii启动子及CDS To detect AvrPii promoter and CDS AvrPii R ACTGTCCGCCGCTCGTTTGG AvrPi9 F ATGCAGTTCTCTCAGATCCTC 342 检测AvrPi9 CDS To detect AvrPi9 CDS AvrPi9 R CTACCAGTGCGTCTTTTCGAC AvrPiz-t F GTTGCGATTATGATCCGTCG 1144 检测AvrPiz-t启动子及CDS To detect AvrPiz-t promoter and CDS AvrPiz-t R GTACTCTAGCAAACGACCGG AvrPia F CAGAGAAACGGACTTGGAGG 1220 检测AvrPia启动子及CDS To detect AvrPia promoter and CDS AvrPia R GGTATACACGTACGGTAGGG AvrPik-CDE F TCCTGCTGCTAACTCCATTC ~1000 检测AvrPik-CDE型启动子及CDS To detect the type of AvrPik-CDE promoter and CDS AvrPik-CDE R GGGTACAGGAATACCAGG AvrPik-D F ACCCTAACTTTTTCGACC 286 检测AvrPik-D型CDS To detect the type of AvrPik-D CDS AvrPik-D R TCAACCAAGCGTAAACCTCG AvrPib F ATGCCGACAATGCGAGGTAT 1598 检测AvrPib启动子及CDS To detect AvrPib promoter and CDS AvrPib R GGACAAGGGAGGCAAATCTAAC