小麦转录因子基因TaWRKY33的耐盐性分析

张惠媛,刘永伟,杨军峰,张双喜,于太飞,陈隽,陈明,周永斌,马有志,徐兆师,付金东

Identification and Analysis of Salt Tolerance of Wheat Transcription Factor TaWRKY33 Protein

ZHANG HuiYuan,LIU YongWei,YANG JunFeng,ZHANG ShuangXi,YU TaiFei,CHEN Jun,CHEN Ming,ZHOU YongBin,MA YouZhi,XU ZhaoShi,FU JinDong

表1 候选基因的BLAST分析结果及其推测的功能

Table 1 BLAST analysis and predicted functions of candidate genes

TaWRKY33的互作蛋白 The proteins interacting with TaWRKY33 GenBank登录号 GenBank accession No. 功能 Functions 烯醇化酶Enolase 乙醇酸氧化酶Glycolate oxidase KC342469 AAB82143 糖酵解过程Glycolytic process 光呼吸反应中的关键酶,能氧化乙醇酸形成乙醛酸 One of the key enzymes in photorespiration where it oxidizes glycolate to glyoxylate 1,5-二磷酸核酮糖羧化酶/加氧酶小亚基 Chloroplast ribulose-1,5-bisphosphate carboxylase/ oxygenase small subunit (rbcS) gene KT288199 主要在固定CO2中对D-核酮糖二磷酸羧化以及对戊糖底物的氧化裂解 To catalyze the primary CO2 fixation step in the reductive pentose phosphate pathway 叶绿素a/b结合蛋白 Chlorophyll a/b-binding protein AAT81763 在光合作用中利用光能同化二氧化碳 To utilizie light energy to assimilate carbon dioxide in photosynthesis ATP合成酶γ亚基ATP synthase gamma subunit ADC33136 与其他亚基组成ATP合成酶 To form the ATP synthase with other subunits 半胱氨酸蛋白酶抑制剂 Cysteine proteinase inhibitor AY062608 抑制蛋白氨基酸肽链内切酶的水解作用,维持细胞蛋白的构象 Protect cells from inappropriate endogenous or external proteolysis and may regulate intra-or extracellular protein breakdown 泛素结合酶2E2 XM_003568254 参与蛋白的降解途径和DNA的修复途径 To be involved in the ubiquitin-mediated protein degradation pathway and the DNA repair pathway 电压依赖性阴离子通道VDAC3 X82148 电压门控离子通道活性Vo ltage-gated anion channel activity 铝胁迫基因AIP ACV44213 受到铝、干旱以及高盐胁迫上调表达 Up-regulated by the imposition of aluminum, high-salt, and drought stress WD40重复蛋白WD40 尿酸氧化酶Urate oxidase 未知蛋白Unknown protein NM_124800 DQ365938 控制植物表皮特征,并且能通过与其他蛋白互作来调控下游基因的表达 Controls plant epidermal traits, and the combination and interaction of these regulatory proteins determine the set of downstream genes to be expressed 参与尿酸合成第一步 This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate 小麦染色体3B基因组片段 Triticum aestivum chromosome 3B, genomic scaffold