黄花蒿提取物对奶牛瘤胃发酵指标的影响
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王丽芳,斯琴毕力格,敖长金
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The Effects of Artemisia annua Extracts on the Rumen Fermentation in Dairy Cows
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WANG LiFang, ,AO ChangJin
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表2 瘤胃氢化菌PCR的引物序列
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Table 2 Primer sequence of biohydrogenation associated bacteria used for real-time PCR
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| 细菌 Target bacterium | 序列(5’ to 3’) Primer sequence | 扩增片段Product size（bp） | 退火温度Annealing temperature (℃) | | 瘤胃总菌 Total bacteria | F:CGGCAACGAGCGCAACCC
R:CCATTGTAGCACGTGTGTAGCC | 147 | 55 | | 溶纤维丁酸弧菌 B.fibrisolvens | F:GCCTCAGCGTCAGTAATCG
R:GGAGCGTAGGCGGTTTTAC | 187 | 55 | | 蛋白溶解梭菌 C.proteoclasticum | F:TCCGGTGGTATGAGATGGGC
R:GTCGCTGCATCAGAGTTTCCT | 185 | 55 |
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