葡萄KEA家族基因的克隆、鉴定及表达分析

王壮伟,王庆莲,夏瑾,王西成,宋志忠,吴伟民

Cloning, Characterization and Expression Analysis of K +/H + Antiporter Genes in Grape

WANG ZhuangWei,WANG QingLian,XIA Jin,WANG XiCheng,SONG ZhiZhong,WU WeiMin

表1 实时荧光定量PCR所用特异性引物

Table 1 Specific primers used for qRT-PCR

基因 Gene 引物 Primer (5’-3’) 扩增产物大小Amplicon size (bp) VvKEA1 F: GTCAATGGCACTAACCCCCT R: TCATAAAAATGATGTACCTGAC 171 VvKEA2 F: GGTACTGTTGCGGTGGTTCT R:TCATGAGAGCCTTGGACCATC 114 VvKEA3 F: GGTGACGACTCCTGTCCTGT R: ACGGAAGCTCTCTCCTCATTC 111 VvKEA4 F: GACTGATGAAGAAGCTTCCCT R: ACCAGCAGCCAAGTAACCAA 121 Ubiquitin F: CCTCATCTTCGCTGGCAAAC R: GGTGTAGGTCTTCTTCTTGCG 133