红铃虫性信息素合成激活肽基因克隆、序列特征及在不同发育阶段的表达分析

许冬,王玲,丛胜波,王金涛,李文静,万鹏

Cloning, Sequence Analysis and Expression of Pheromone Biosynthesis Activating Neuropeptide (PBAN) Gene in Different Development Stages of Pectinophora gossypiella

XU Dong,WANG Ling,CONG ShengBo,WANG JinTao,LI WenJing,WAN Peng

表1 PgosPBAN扩增所用引物

Table 1 The primers for PgosPBAN amplification

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Primer use PgosPBAN-F CTBTGGTTCGGYCCYMGACTMGG cDNA克隆 cDNA cloning PgosPBAN-R CATSGTBGKSCCBAGCCTKGGBGAGAAGT 3′ PgosPBAN-Outer CAAACCTACCTCCGTCTTCTT 3′端cDNA扩增 3′-cDNA end amplification 3′ PgosPBAN-Inner CGCCCTAAAATACTACTACGAC 5′ PgosPBAN-Outer GTCAGCTAACCTCCTTCCGAG 5′端cDNA扩增 5′-cDNA end amplification 5′ PgosPBAN-Inner CACTCTCGTATCGCTTCCTCT PgosPBAN-O-F GCAACAGTAAATCGTAGAAACA 开放阅读框扩增 ORF amplification PgosPBAN-O-R GATGAGCATAAACCAGCCAAT PgosPBAN-Q-F GGCAAGCGTTCTTTCCATCC 实时荧光定量PCR qRT-PCR PgosPBAN-Q-R GCCTCGTCATCAGCCTGTG ACS1 CACCGTGCCCATCTATGAAGG ACR1 GACGATTTCCCTCTCAGCGGT