一个甘蔗Ⅱd类WRKY转录因子基因的克隆和表达分析

张旭,凌辉,刘峰,黄宁,王玲,毛花英,李聪娜,汤翰臣,苏炜华,苏亚春,阙友雄

Cloning and Expression Analysis of a Ⅱd Sub-Group WRKY Transcription Factor Gene from Sugarcane

ZHANG Xu,LING Hui,LIU Feng,HUANG Ning,WANG Ling,MAO HuaYing,LI CongNa,TANG HanChen,SU WeiHua,SU YaChun,QUE YouXiong

图7 甘蔗ScWRKY6转录激活活性分析 SD/-Trp：色氨酸营养缺陷型平板培养基;SD/-Trp (+X-α-Gal)：色氨酸营养缺陷型平板培养基（添加5-溴-4-氯-3-吲哚-α-D-半乳糖苷）;SD/-Trp (+X-α-Gal+AbA)：色氨酸营养缺陷型平板培养基（添加5-溴-4-氯-3-吲哚-α-D-半乳糖苷和金担子素A）

Fig. 7 The transactivation activity assay of ScWRKY6 in sugarcane SD/-Trp: Synthetic dropout medium plate; SD/-Trp(+X-α-Gal): Synthetic dropout medium plate without tryptophan (plus 5-bromo-4-chloro-3-indoxyl-α-D- galactopyranoside); SD/-Trp(+X-α-Gal+AbA): Synthetic dropout medium plate without tryptophan (plus 5-bromo-4-chloro-3-indoxyl-α-D-galactopyranoside and aureobasidin A)