一个甘蔗Ⅱd类WRKY转录因子基因的克隆和表达分析

张旭,凌辉,刘峰,黄宁,王玲,毛花英,李聪娜,汤翰臣,苏炜华,苏亚春,阙友雄

Cloning and Expression Analysis of a Ⅱd Sub-Group WRKY Transcription Factor Gene from Sugarcane

ZHANG Xu,LING Hui,LIU Feng,HUANG Ning,WANG Ling,MAO HuaYing,LI CongNa,TANG HanChen,SU WeiHua,SU YaChun,QUE YouXiong

图6 ScWRKY6在本氏烟中下表皮细胞中的亚细胞定位 图片采用绿色荧光、明场、绿色荧光和明场叠加这3个视野拍摄。35S::GFP：携带空载pCAMBIA1300-GFP的农杆菌菌株。35S::ScWRKY6::GFP：携带重组载体pCAMBIA1300-ScWRKY6-GFP的农杆菌菌株。红色箭头1、2和3分别代表细胞膜、细胞质和细胞核。比例尺=25 μm

Fig. 6 Subcellular localization of ScWRKY6 in Nicotiana benthamiana lower epidermal cells The images are taken by green fluorescence, visible light, merged green fluorescence and visible light. 35S::GFP: The Agrobacterium tumefaciens strain carrying the empty vector pCAMBIA1300-GFP. 35S::ScWRKY6::GFP: The A. tumefaciens strain carrying the recombinant vector pCAMBIA1300-ScWRKY6- GFP. Red arrows 1, 2 and 3 indicate plasma membrane, cytoplasm and nucleus, respectively. Scale bar = 25 μm