一个甘蔗Ⅱd类WRKY转录因子基因的克隆和表达分析
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张旭,凌辉,刘峰,黄宁,王玲,毛花英,李聪娜,汤翰臣,苏炜华,苏亚春,阙友雄
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Cloning and Expression Analysis of a Ⅱd Sub-Group WRKY Transcription Factor Gene from Sugarcane
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ZHANG Xu,LING Hui,LIU Feng,HUANG Ning,WANG Ling,MAO HuaYing,LI CongNa,TANG HanChen,SU WeiHua,SU YaChun,QUE YouXiong
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表1 引物序列及用途
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Table 1 Sequences of primers and their usage
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| 引物名称Primer name | 引物序列Primer sequence (5°-3°) | 用途Usage | | ScWRKY6 | F: GCTGCTGAGGGAGTTGCATA | 扩增全长 Full length amplification | | R: AAGCAGGGCCAAACATCTCA | | GFP-ScWRKY6 | F: GGGGTACCATGGAGGAAGTGGAGGAGGC | 亚细胞定位 Subcellular localization | | R: GCTCTAGACACCTGTGCTGACTGAGTTGGC | | BD-ScWRKY6 | F: CATGCCATGGAGATGGAGGAAGTGGAGGAGGC | 转录激活 Transactivation | | R: CGCGGATCCCACCTGTGCTGACTGAGTTGGC | | qScWRKY6 | F: GCGAGGTCCTTCTTGTCGTC | 实时荧光定量PCR qRT-PCR | | R: CGTTCACCGGATCGCTCATT | | CAC | F: ACAACGTCAGGCAAAGCAAA | 内参基因 Actin gene | | R: AGATCAACTCCACCTCTGCG | | CUL | F: TGCTGAATGTGTTGAGCAGC | 内参基因 Actin gene | | R: TTGTCGCGCTCCAAGTAGTC |
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