鸭胚胎发育中后期胸肌发育阻滞的RNA-seq分析

刘宏祥,徐文娟,朱春红,陶志云,宋卫涛,章双杰,李慧芳

RNA-seq Analysis on Development Arrest of Duck Pectoralis Muscle During Semi-Late Embryonic Period

LIU HongXiang,XU WenJuan,ZHU ChunHong,TAO ZhiYun,SONG WeiTao,ZHANG ShuangJie,LI HuiFang

表1 用于qRT-PCR验证的基因及其引物

Table 1 Genes and primers designed for qRT-PCR validating

基因 Gene 上游引物 Up-primer 下游引物 Lower-primer 产物长度 Product length (bp) 退火温度 Temperature (℃) F16P1 CCCTAAAGGAAAGCTGAGAC CTCGGGCACTATATCCAGTA 115 60 COL1A1 GCCAACGAAATCGAGATCAG CGTCTTTGTCGTCTTGTACT 126 60 WIPI1 GAAATCCCAGATGTTTACATCG TCCGTCCCTTTCTTGAAGT 113 60 ARMC3 TTGGCATTGGCTGTAGTTTAG TACTCTTGAGGAGGAAGCAGAA 115 60 CEA20 TCTCCGAGCCAGAAATCC TCTTCCACCAGTACACGTC 105 60 GLIS3 ATGACGCAGAGACAAACT TTGTGTTGTACGTTCTTCTGAG 188 60 SRSF4 CTCTTACTCCAGAAGCCGA GATCTACTCTTGGAGCGAC 105 60 LAMC3 GAGGCCCAGAAGAAGATCA ACAACCTGTGCCCTCTTA 131 60 TNNI1 CTGCACGAGAAGGTTGAG GCAGGTCAAGCACTTTGAT 109 60 TNNT2 TCTCCAACATGCTGCATT CTGAGGTGGTCGATGTTC 130 60 TNNC1 ATGCTGCTTTAACTGGAATG CACCACAGGGTGGAAATC 179 60 MYOZ1 CAGAAGATTCAGTCTCACAAGT AGGTCTTTATCCAAGCCAC 103 60 MYBPH TCATGGGCAACACCTACTC GGATCTTCTCTGGCTGGTAA 129 60 COEA1 CTTCCTGCCAGCAATTAC AGCCAAACACTTCCATCATA 134 60 KCRS GACCAGTGCATCCAAACC TCTCAGCAAACACCTCGTAG 103 60 DEP1A ATCTGTTGTTTGTTGCTTCC TGTATCATCAAAGAGCGTGTG 134 60 ACTB* TGAGAGTAGCCCCTGAGGAGCAC TAACACCATCACCAGACTCCATCAC 198 60 GAPDH* CTTCGGAATAGGGAGGAGAC CGGAGATGATGACACGCTTA 131 60