苹果赤霉素氧化酶基因MdGA2ox8的克隆及功能分析
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李飞鸿,侯应军,李雪涵,余心怡,渠慎春
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Cloning and Function Analysis of Apple Gibberellin Oxidase Gene MdGA2ox8
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LI FeiHong,HOU YingJun,LI XueHan,YU XinYi,QU ShenChun
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表1 苹果MdGA2ox8克隆、表达及功能分析所用引物
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Table 1 Primers used for cloning, expression and functional analysis of MdGA2ox8
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| 引物名 Primer name | 引物序列Primer sequence (5′-3′) | 产物Product | | MdGA2ox8-F1 | ATGTCAATGAAGATTCTA | 开放阅读框
ORF | | MdGA2ox8-R1 | TTATACGACAAATCTAGG | | MdGA2ox8-F2 | C/GAGCTC/ATGTCAATGAAGATTCTA | 酶切位点SacⅠ/BamHⅠ
Restriction site SacⅠ/BamHⅠ | | MdGA2ox8-R2 | CG/GGATCC/TTATACGACAAATCTAGG | | GA2ox8-RT-F | GAGGAGTGCATGACGGAGAT | 特异扩增
Special amplification | | GA2ox8-RT-R | ATGTGGCAGAAGGAGTTCCC | | NtGA3ox1-RT-F | ATCGACCTCGACAACTACATCA | 特异扩增
Special amplification | | NtGA3ox1-RT-R | CAGGAGAACGAGCAGCCTTA | | NtGA3ox2-RT-F | GTAGTGAACCGAACCCGACA | 特异扩增
Special amplification | | NtGA3ox2-RT-R | GCGCAAAGCTGAAAAGACGA | | NtTubulin-F | AGATGTTCCGTCGTGTCAGTG | 烟草内参基因
Tubulin in tobacco | | NtTubulin-R | TGCTTCCTCTTCATCCTCATATCC | | MdTubulin-F | AGGATGCTACAGCCGATGAG | 苹果内参基因
Tubulin in apple | | MdTubulin-R | GCCGAAGAACTGACGAGAATC |
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