AtNEK6在棉花旱盐胁迫响应中的功能分析

范鑫,赵雷霖,翟红红,王远,孟志刚,梁成真,张锐,郭三堆,孙国清

Functional Characterization of AtNEK6 Overexpression in Cotton Under Drought and Salt Stress

FAN Xin,ZHAO LeiLin,ZHAI HongHong,WANG Yuan,MENG ZhiGang,LIANG ChengZhen,ZHANG Rui,GUO SanDui,SUN GuoQing

图2 AtNEK6促进了转基因棉花的生长发育 A：野生型植株和转基因植株的表型,Bar=10 cm;B：野生型植株和转基因植株的第三叶至第十叶,Bar=5 cm;C：野生型植株和转基因植株的株高;D：野生型植株和转基因植株的叶面积,1—8：第一片真叶到第八片真叶;E：扫面电镜观察野生型植株和转基因植株的叶表皮细胞,Bar=50 μm;F：野生型植株和转基因植株的表皮细胞面积;G：细胞周期和生长发育相关基因的表达量检测。*表示在P<0.05水平差异显著。下同

Fig. 2 AtNEK6 promotes the growth of transgenic cotton A: Growth phenotype of plant from WT and transgenic lines, Bar=10 cm; B: Morphology of leaves from WT and transgenic lines, Bar=5 cm; C: Height of plant from WT and transgenic lines; D: The difference of leaf area between WT and transgenic lines, 1-8: The first true leaf to the eighth true leaf; E: Leaf epidermal cells of WT and transgenic lines under SEM, Bar=50 μm; F: Leaf epidermal cell area of WT and transgenic lines; G: Transcript levels of cell cycle-related/ growth-related genes in WT and transgenic lines revealed by qRT-PCR. * indicate significant difference at P<0.05 level, respectively. The same as below