猪瘟病毒中等致病力毒株在体内的动态分布

孙骏翔,张乾义,徐和敏,王团结,徐璐,邹兴启,朱元源,李翠,夏应菊,徐嫄,陈锴,张玉杰,赵启祖,王琴

Dynamic Distribution of Classical Swine Fever Virus in vivo After Infection by Intermediate Virulent Strains

JunXiang SUN,QianYi ZHANG,HeMin XU,TuanJie WANG,Lu XU,XingQi ZOU,YuanYuan ZHU,Cui LI,YingJu XIA,Yuan XU,Kai CHEN,YuJie ZHANG,QiZu ZHAO,Qin WANG

表1 各组织器官的预处理液煮沸时间及蛋白酶消化时间

Table 1 The optimization of boiling and proteinase digestion time for pre-treated tissue samples

组织名称 Tissue 预处理液处理时间 Preconditioning liquids work time (min) 蛋白酶消化时间 Proteinase K work time (min) 肺脏Lung 10 20 十二指肠 Duodenum 10 10 回盲瓣 Ileocecal valve 10 10 胰腺Pancreas 10 10 脾脏Spleen 20 10 肾脏Kidney 20 20