利用胚挽救技术创制无核抗寒葡萄新种质

赵雅楠,骆强伟,王跃进

Breeding for Grape Germplasm Involved in Seedlessness with Cold-Resistant Using Embryo Rescue

YaNan ZHAO,QiangWei LUO,YueJin WANG

表3 不同发育培养基组分对不同杂交组合胚挽救成苗的影响

Table 3 Effect of different ovule development media on embryo rescue of different crosses

杂交组合 Cross combination 培养基 Culture medium 胚珠数 Number of ovule 发育胚 Embryos developed 胚萌发成苗 Plantlet formation Number % Number % 无核白×双优 Thompson Seedless × Shuangyou 基本培养基 Basal medium MM3 135 26 19.26a 11 8.15a ER 195 26 13.33b 7 3.59b 红宝石无核×北醇 Ruby Seedless × Beichun MM3 150 69 46.00a 26 17.33a ER 120 42 35.00b 11 9.17b 昆香无核×双优 Kunxiang Seedless × Shuangyou 添加不同氨基酸的培养基 Medium with different amino acids MM3+2.5 mmol·L-1半胱氨酸Cysteine 150 62 41.33c 28 18.67ab MM3+2.5 mmol·L-1天冬酰胺Asparagine 140 67 47.86bc 30 21.43a MM3+2.5 mmol·L-1谷氨酰胺Glutamine 129 77 59.69a 27 20.93a MM3+2.5 mmol·L-1丝氨酸Serine 165 88 53.33ab 22 13.33c MM3 141 64 45.39bc 23 16.31bc 红宝石无核×北醇 Ruby Seedless × Beichun MM3+2.5 mmol·L-1半胱氨酸Cysteine 435 205 47.13b 50 11.49c MM3+2.5 mmol·L-1天冬酰胺Asparagine 70 39 55.71a 15 21.43a MM3+2.5 mmol·L-1谷氨酰胺Glutamine 360 165 45.83b 80 22.22a MM3+2.5 mmol·L-1丝氨酸Serine 340 151 44.41b 64 18.82b MM3 150 69 46.00b 26 17.33b