植物RNA结合蛋白研究进展

张在宝,李婉杰,李九丽,张弛,胡梦辉,程琳,袁红雨

The Research Progress of Plant RNA Binding Proteins

ZaiBao ZHANG,WanJie LI,JiuLi LI,Chi ZHANG,MengHui HU,Lin CHENG,HongYu YUAN

表1 植物RNA结合蛋白的分类、结构特征及主要功能

Table 1 Classification, structural characteristics and main functions of plant RBPs

分类 Classification 结构特征 Structural characteristics 生理功能 Physiological function SR蛋白 SR proteins N端：含1个或2个RRM结构域; C端：富含SR的结构域 N-terminal: Containing 1 or 2 RRM domain; C-terminal: SR-rich domain 参与mRNA剪接、输出或翻译,调节生长发育及细胞增殖;响应高温和低温等非生物胁迫 Participating in mRNA splicing, export or translation, regulating plant growth, development and cell proliferation; Responding to abiotic stresses such as heat and cold stresses GR-RBPs (Ⅳ of GRPs） N端：含RRM或CSD结构域; C端：存在富含甘氨酸区域 N-terminal: Containing RRM or CSD domain; C-terminal: Containing the regions of glycine rich 作为RNA分子伴侣,响应干旱、高盐和低温等胁迫;调节植物生物钟,介导开花等过程 As RNA molecular chaperone, responding to drought, salt and cold stresses; Regulating plant circadian clock, mediating plants flowering, etc. RZs (IVb of GRPs) N端：含RRM结构域; C端：富含甘氨酸区域且其间散布CCHC型锌指基序 N-terminal: Containing RRM domain; C-terminal: The region rich in glycine and interspersed with CCHC-type zinc finger motifs 响应低温和干旱等胁迫;与SR等蛋白互作参与mRNA剪接,促进种子萌发、幼苗生长和开花等植物发育 Responding to cold and drought stresses; interacting with SR and other proteins to participate in mRNA splicing, promote seed germination, seedling growth and flowering, etc. CSDPs (IVc of GRPs) N端：含一个CSD结构域; C端：含一个富含甘氨酸区域且其间散布CCHC型锌指基序 N-terminal: Contains a CSD domain; C-terminal: Containing a glycine-rich region interspersed with CCHC-type zinc finger motifs 正/负调控植物的胁迫耐受性、生长发育等 Positively/negatively regulating the plant stress tolerance, growth and development, etc. PPR蛋白 PPR proteins 由大约35个氨基酸序列组成的串联重复基序 The tandem repeat motif consisting of approximately 35 amino acid sequences 主要参与线粒体和叶绿体的RNA代谢,调节光合作用、呼吸作用、胚胎发生等生理和生长发育过程;响应逆境胁迫 Mainly participating in the RNA metabolism of mitochondrial and chloroplast, regulating photosynthesis, respiration, embryogenesis and other physiological and growth processes; Responsing to adversity stress DEAD-box RHs 包含Q、Ⅰ、Ⅱ（DEAD）、Ⅲ、Ⅳ、Ⅴ和Ⅵ结构域 Containing Q, Ⅰ, Ⅱ(DEAD), Ⅲ, Ⅳ, Ⅴ and Ⅵ domains 催化RNA分子二级结构解旋,参与RNA代谢,调节植物各种细胞代谢途径、生长发育;响应低温、干旱等非生物胁迫 Catalyzing the secondary structure of RNA molecules to unwind, participating in RNA metabolism, regulating various cellular metabolic pathways, growth and development; Responding to cold, drought and other abiotic stresses RNA分子伴侣 RNA molecular chaperone 具有RNA伴侣蛋白活性的RBP,结构特征多样 The structural features of the RBPs with RNA chaperone activity are diverse 参与非生物胁迫下RNA的折叠反应,提高植物的胁迫耐受性;参与RNA代谢,调节叶绿体相关生物合成等过程 Participating in the folding reaction of RNA under abiotic stresses, thereby improving the stress tolerance of plants; participating in RNA metabolism, regulating the process of chloroplast-related biosynthesis, etc.