意大利蜜蜂工蜂中肠发育过程中长链非编码RNA的 差异表达分析

郭睿, 耿四海, 熊翠玲, 郑燕珍, 付中民, 王海朋, 杜宇, 童新宇, 赵红霞, 陈大福

Differential Expression Analysis of Long Non-Coding RNAs During the Developmental Process of Apis mellifera ligustica Worker’s Midgut

Rui GUO, SiHai GENG, CuiLing XIONG, YanZhen ZHENG, ZhongMin FU, HaiPeng WANG, Yu DU, XinYu TONG, HongXia ZHAO, DaFu CHEN

表2 RT-PCR与RT-qPCR引物信息

Table 2 Information of primers for RT-PCR and RT-qPCR

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 1-F GGCTGAAGATTTCGGATTC 1-R AGAAGGAGGCAAGGAGGAT 2-F GCAAAGACGGAAAGATGG 2-R CCGATGAGTGTGTTCAGTTT 3-F GCCTGTTAGCCATAGTAAGACG 3-R AGAGTGTTGAGCAGCGTTG 4-F CGAGGATGAGCAACTGACA 4-R GCTACGAGCCAGAAGTCTTT 5-F CGCAGTAATGAAAGCATAGG 5-R CGCATCGTGTAACCATAAGA 6-F CCTCTTGGAGATTCCGATACAG 6-R CGTTACCACCATTCAACACG 7-F CCTCTTGGAGATTCCGATACAG 7-R ACCATTCAACACGAGCACC 8-F CCTCTTGGAGATTCCGATACAG 8-R ACCACCATTCAACACGAGC RE1-F GTTGCTCAAACATCCGAGT RE1-R CGTTCCATCTTCCTCCAAG RE2-F TCGTATTCTACAGGGCTTGG RE2-R TCGCTTCCTTCGTTTAGG RE3-F GGTTTACTATGCTCCGACGA RE3-R GGTGATACCGATGGACTCA RE4-F AGCCAACAGGTGAAATGTG RE4-R AGGTGTCAGACTGCGGTAA RE5-F CGTTTCTCGTGCTGCTCTCT RE5-R AGATGCCACACTTGGATGG Actin-F CACTCCTGCTATGTATGTCGC Actin-R GGCAAAGCGTATCCTTCA