FSH处理对猪颗粒细胞中类固醇合成酶基因的表达 及其调控区组蛋白H3修饰的影响
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张金璧, 姚望, 潘增祥, 刘红林
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Effects of FSH Treatment on Steroidogenic Enzymes Expression and Histone H3 Modification in Pig Granulosa Cells
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JinBi ZHANG, Wang YAO, ZengXiang PAN, HongLin LIU
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| 表1 PCR引物及反应条件 |
| Table 1 Primer and PCR reaction conditions |
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| 基因Gene | 登陆号Acc. No. | 引物(5'- 3')Primer (5'- 3') | 产物长度Product length（bp） | | STAR | XM_005671764.2 | F: GCTGAGCCCTTTCGTGTCTA | 140 | | R: CATAGGACCTGCCGTGTCTG | | CYP11A1 | NM_214427.1 | F: AGACACTGAGACTCCACCCCA | 110 | | R: GACGGCCACTTGTACCAATGT | | HSD3B | NM_001004049.1 | F: CCCAGTGTTTTCTGGTTCCT | 131 | | R: TTCTCCTCCAGCAACAAGTG | | CYP19A | NM_214431.1 | F: TGCTGGACACCTCTAACAA | 264 | | R: TCAACTCAGTGGCGAAAT | | FSHR | NM_214386.3 | F: GAATTGAAAAGGCCAACAAC | 214 | | R: CTTTCAAAACTTAGTCCCACG | | LHR | NM_214449.1 | F: ACTCCAATGTGCTCCCGAAC | 222 | | R: AGTAGCAGGTAGAGCCCCAT | | XIAP | NM_001097436.1 | F: ACTGGCCAGACTATGCTCAC | 107 | | R: CAGTTTCCCGCCACAACAAA | | FasL-2 | AY033634 | F: CCACCACTCCTGCCATCAA | 135 | | R: CAGCCCCAATCCAACCA | | GAPDH | AF017079 | F: GGACTCATGACCACGGTCCAT | 220 | | R: TCAGATCCACAACCGACACGT | | STAR-ChIP | chr15:55501189
-55501271 | F: GAGCAACATTCCTCCCTAGACT | 83 | | R: CAGCCCCAATCCAACCA | | HSD3B-ChIP | chr4:111565035
-111565175 | F: GCCGGCTGTGACTATTTGGA | 141 | | R: TGCTTCAAGGACTGTGTCCC | | CYP19A-ChIP | chr1:133903003
-133903130 | F: ACCCTTAACTCAAAAGAACCCA | 128 | | R: AATTTTGCAGTGGCGGGATT |
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