大豆GmWRKY148的克隆与功能分析
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王莎莎, 崔晓霞, 黄颜众, 轩慧冬, 郭娜, 邢邯
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Cloning and Functional Analysis of the GmWRKY148 in Soybean
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ShaSha WANG, XiaoXia CUI, YanZhong HUANG, HuiDong XUAN, Na GUO, Han XING
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| 附表1 试验所用引物序列 |
| Table S1 Primers used in this study |
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| 基因Gene | 引物序列
Sequence of primer (5'-3') | 引物用途
Function of primer | | GmWRKY148 | F：GCAGCTTCTAACTCTGGCGA | GmWRKY148克隆
Cloning of GmWRKY148 | | R：TTTATTATAAGTGATAAGATCTACAAGCAA | | GmWRKY148-MQ | F：CGGGGTACCATGCCTTTCCGAAATTCCAATATGA | | R：CGCGGATCCTCATATTTCATTTGGATATTGAGGA | | GmWRKY148-2 | F：ATGCACACAACCCACTTGCT | GmWRKY148实时荧光定量PCR
qRT-PCR of GmWRKY148 | | R：CAGATGAAGAGTTTCTCTTC | | GmEF1β | F：GTTGAAAAGCCAGGGGACA | 疫霉积累量实时荧光定量内参
Reference gene in qRT-PCR | | R：TCTTACCCCTTGAGCGTGG | | GmActin | F：GGTGGTTCTATCTTGGCATC | 实时荧光定量PCR内参
Reference gene in qRT-PCR | | R：CTTTCGCTTCAATAACCCTA | | pBinGFP2 | F：AAGACCCCAACGAGAAGC | pBinGFP2载体引物
Primer of vector | | R：GAACCCTAATTCCCTTATCTG |
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