番茄褪绿病毒实时荧光定量PCR检测技术的建立

丁天波, 刘晓蓓, 李洁, 魏可可, 褚栋

Development of a Real-Time Fluorescent Quantitative PCR Method for the Detection of Tomato chlorosis virus and Its Application

TianBo DING, XiaoBei LIU, Jie LI, KeKe WEI, Dong CHU

表1 实时荧光定量PCR检测单头烟粉虱体内ToCV含量

Table 1 Detection and quantitation of ToCV in single B. tabaci by RT-qPCR

样品号 Sample number 组1 Group 1 组2 Group 2 组3 Group 3 Ct值 Ct value ToCV含量 Concentration of ToCV (copies/μL) Ct值 Ct value ToCV含量 Concentration of ToCV (copies/μL) Ct值 Ct value ToCV含量 Concentration of ToCV (copies·μL-1) 1 30.37 2238 30.35 2270 31.35 1134 2 28.55 7910 31.3 1174 32.16 646 3 30.90 1550 27.69 14363 29.65 3688 4 30.53 2003 29.84 3233 29.76 3418 5 29.85 3211 30.68 1805 28.61 7588 6 29.95 2996 28.68 7228 26.86 25542 7 27.59 15395 28.34 9151 30.4 2192 8 31.02 1426 31.12 1330 27.83 13034 Nc1 No Ct — No Ct — No Ct — Nc2 No Ct — No Ct — No Ct —