%0 Journal Article %A LIU Jiao %A ZHANG Xue-yao %A WU Hai-hua %A MA Wen %A ZHU Wen-ya %A Kun-Yan ZHU %A MA En-bo %A ZHANG Jian-zhen %T Characteristics and roles of cytochrome b5 in cytochrome P450-mediated oxidative reactions in Locusta migratoria %D 2020 %R 10.1016/S2095-3119(19)62827-3 %J Journal of Integrative Agriculture %P 1512-1521 %V 19 %N 6 %X
Cytochrome b5 (Cyt-b5) is a small heme protein and known to be involved in a wide range of biochemical transformations, including cytochrome P450 monooxygenase (CYP)-mediated metabolism of endogenous and exogenous compounds.  Studies on Cyt-b5 are more concentrated in mammals, but are relatively rare in insects.  The characteristics and function of Cyt-b5 from Locusta migratoria have not been described yet.  We sequenced the full-length cDNA sequence of Cyt-b5 from L. migratoria (LmCyt-b5) by reverse transcription-PCR (RT-PCR) based on locust transcriptome database.  The phylogenetic analysis showed that LmCyt-b5 was closely related to the Cyt-b5 from Blattodea.  LmCyt-b5 was highly expressed in ovary, Malpighian tubules, midgut, gastric caeca, and fat bodies.  Silencing of LmCyt-b5 had no effect on the susceptibility of L. migratoria to four different insecticides.  Suppression of LmCyt-b5 or silencing of both LmCyt-b5 and LmCPR did not significantly change the total CYP activity toward the substrate 7-ethoxycoumarin (7-EC).  However, coexpression of LmCYP6FD1 with LmCPR and LmCyt-b5 together in Sf9 cells by using Bac-to-Bac baculovirus expression system significantly increased the catalytic activity of LmCYP6FD1 toward 7-EC as compared with the coexpression of LmCYP6FD1 with cytochrome P450 reductase (LmCPR) or LmCyt-b5 separately.  These results suggest that LmCyt-b5 plays an important role in the catalytic reaction of LmCYP6FD1 toward 7-EC in our in vitro experiments.  Further study is needed to clarify the role of LmCyt-b5 in CYP-mediated catalytic reactions in L. migratoria.
 
%U https://www.chinaagrisci.com/Jwk_zgnykxen/EN/10.1016/S2095-3119(19)62827-3