%0 Journal Article %A GUO Huan-cheng %A REN Zhao-wen %A DING Mei-ming %A XIAO Wan-jun %A PENG Peng %A HE Biao %A FENG Ye %A LIU Yan %A LI Xing-yu %A CAI Jian-qiu %A ZHANG Bi-kai %A LUO Qing-hua %A TU Chang-chun %T Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China %D 2019 %R 10.1016/S2095-3119(19)62650-X %J Journal of Integrative Agriculture %P 2133-2140 %V 18 %N 9 %X
Hybrid wild boar husbandry is an important component of livestock production in Northeast China.  However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance.  The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China.  Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces.  ELISA detected serum antibodies against classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and Japanese encephalitis virus (JEV), but not against African swine fever virus (ASFV), with PCV2 having the highest seropositive rate (87.2–100% in different farms).  RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1% (56/169) of blood samples and 32.8% (20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars.  Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively. 
%U https://www.chinaagrisci.com/Jwk_zgnykxen/EN/10.1016/S2095-3119(19)62650-X