%0 Journal Article %A XU Yuan %A ZHANG Ai-ling %A ZHANG Zhe %A YUAN Xiao-long %A CHEN Zan-mou %A ZHANG Hao %A LI Jia-qi %T MicroRNA-34c regulates porcine granulosa cell function by targeting forkhead box O3a %D 2017 %R 10.1016/S2095-3119(16)61582-4 %J Journal of Integrative Agriculture %P 2019-2028 %V 16 %N 09 %X Granulosa cells (GCs) are somatic cells of ovary, the behaviors of GCs are important for ovarian function.  MicroRNAs (miRNAs) are a class of endogenous 18–24 nucleotide (nt) non-coding RNAs, some of which have been shown to be important regulators of GCs function.  miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types.  However, the roles of miR-34c in GCs function remain unknown.  In this study, we used Annexin V-FITC and EdU assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs.  Dual-luciferase reporter assays, quantitative real-time PCR (qRT-PCR) and Western blotting identified Forkhead box O3a (FoxO3a) as a direct target gene of miR-34c.  The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs.  In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a. %U https://www.chinaagrisci.com/Jwk_zgnykxen/EN/10.1016/S2095-3119(16)61582-4