本研究旨在利用拉曼光谱学方法鉴别花生油产地，并建立稳健的鉴别模型，进一步筛选出与产地密切相关的特征光谱。对来自不同省份和同一省份不同城市的159个花生油样品进行拉曼光谱测定，获得的数据进行了逐步线性判别分析分析(SLDA)，k-最近邻分析(k-NN)和多因素方差分析等。结果表明，基于全光谱的样本识别率达到90%以上。花生油的产地、品种及其互作对拉曼光谱影响显著，筛选出1400-1500 cm^-1和1600-1700 cm^-1为受品种影响较小的产地特征光谱。结合产地特征光谱建立的SLDA最佳分类模型能够快速、准确地识别花生油的产地。

Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) catalyses phosphoenolpyruvate (PEP) to yield oxaloacetate, which is involved in protein biosynthesis. Pyruvate kinase (PK; EC 2.7.1.40) catalyzes PEP to yield pyruvate, which is involved in fatty acid synthesis. In this study, five PEPC genes (AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5) from peanut have been cloned. Using a quantitative real-time RT-PCR approach, the expression pattern of each gene was monitored during the seed development of four peanut varieties (E11, Hebeigaoyou, Naihan 1, and Huayu 26). It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging (DAP); whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou. In Naihan 1 and Huayu 26, the expression levels of the five genes remained relatively high in the first stage. The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development. The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter. These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.