水稻叶片表皮毛是由表皮细胞分化发育形成，表皮毛在植物的抗逆以及防止紫外直射等过程中都具有重要的作用，但关于水稻毛状体发育的研究还存在很大未知。在本研究中，我们对野生型籼系水稻西大1B进行EMS (ethylmethane sulfonate) 诱导，通过表型观察分析筛选出了毛状体发育缺陷突变体，将其命名为lhl1 (Less Hairy Leaf 1)。我们对其进行了基因定位和图位克隆，将其定位在2号染色体两个分子标记的70 kb的区间内，通过基因测序将LOC_Os02g25230确定为候选基因。之后我们构建了干涉以及超表达株系，扫描电镜观察分析发现LHL1-RNAi叶片同lhl1一样存在毛状体发育缺陷，但LHL1-OE株系叶片表面的毛状体形态与野生型相似，但数目大大增加。qRT-PCR分析发现，与野生型相比，突变体lhl1中正向调控毛状体发育相关的基因表达下调。对生长素相关基因定量分析发现，突变体hl7中生长素相关基因的表达严重下调，进一步通过激素响应分析发现HL7的表达受到生长素的诱导，证明了HL7影响水稻叶片毛状体的发育可能与生长素途径有关。

Increasing attentions have been paid to mineral concentration decrease in milled rice grains caused by CO2 enrichment, but the mechanisms still remain unclear. Therefore, mineral (Ca, Mg, Fe, Zn and Mn) translocation in plant-soil system with a FACE (Free-air CO2 enrichment) experiment were investigated in Eastern China after 4-yr operation. Results mainly showed that: (1) elevated CO2 significantly increased the biomass of stem and panicle by 21.9 and 24.0%, respectively, but did not affect the leaf biomass. (2) Elevated CO2 significantly increased the contents of Ca, Mg, Fe, Zn, and Mn in panicle by 61.2, 28.9, 87.0, 36.7, and 66.0%, respectively, and in stem by 13.2, 21.3, 47.2, 91.8, and 25.2%, respectively, but did not affect them in leaf. (3) Elevated CO2 had positive effects on the weight ratio of mineral/biomass in stem and panicle. Our results suggest that elevated CO2 can favor the translocation of Ca, Mg, Fe, Zn, and Mn from soil to stem and panicle. The CO2-led mineral decline in milled rice grains may mainly attribute to the CO2-led unbalanced stimulations on the translocations of minerals and carbohydrates from vegetative parts (e.g., leaf, stem, branch and husk) to the grains.