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1. 高效氯氟氰菊酯微囊的光敏性和粒径精准组合协同作用产生了更好的杀虫效果
GAO Yue, LUO Jian, SUN Yue, ZHANG Hua-wei, ZHANG Da-xia, LIU Feng, MU Wei, LI Bei-xing
Journal of Integrative Agriculture    2023, 22 (5): 1477-1488.   DOI: 10.1016/j.jia.2022.08.081
摘要192)      PDF    收藏

本研究制备了不同粒径的聚脲高效氯氟氰菊酯微囊均有一定程度的塌陷,小粒径微囊塌陷更明显。它们的平均粒径分别为1.38 μm ( MC-S )5.13μm ( MC-M )10.05 μm ( MC-L ),囊壳厚度分别为39.650.3150.1 nm。小粒径微囊具有更快的释放性能;与MC-MMC-L相比,MC-S组对小地老虎的生物活性更高,对花生叶片的亲和力更好(由耐雨水冲刷可知);微囊均表现出随光照增强释放加快的趋势;与EC组相比,微囊降解都较慢,其中MC-L降解最慢。为了获得既具有速效性又具有持效期的微囊,将MC-S、MC-MMC-L2:11:11:2的比例两两混合花生叶面喷雾场景下,9个供试微囊组合中MC-S:MC-L1:2混合药效最好,持效期比乳油延长3倍。因此,不同粒径微囊精准组合可以调控农药的防治效果,为更好地利用农药提供策略。

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2. JIA-2021-0833 灌浆结实期水分调节通过对弱势粒淀粉合成的改善缓解高温胁迫对粳稻籽粒品质的危害
TONG Hui, DUAN Hua, WANG Sheng-jun, SU Jing-ping, SUN Yue, LIU Yan-qing, TANG Liang, LIU Xue-jun, CHEN Wen-fu
Journal of Integrative Agriculture    2022, 21 (10): 3094-3101.   DOI: 10.1016/j.jia.2022.07.012
摘要208)      PDF    收藏
温度和水分是影响水稻产量和品质形成的重要因子,灌浆结实期适度的干旱能够有效的缓解高温胁迫对水稻造成的伤害,但有关其淀粉合成的调控机理尚不清楚。为探明灌浆结实期高温与干旱对水稻淀粉合成的影响及其生理机制,本试验研究了4个不同处理下(对照(CK)、高温(HT)、适度干旱(WS)、高温干旱(HT+WS))常规粳稻淀粉相关酶活性及其基因的表达水平。结果表明高温和干旱对籽粒灌浆的影响主要发生在弱势粒中,并通过调节参与淀粉合成相关酶活性及其主效基因的表达水平,进而影响稻米淀粉的合成。高温和干旱有拮抗作用,适度干旱能够改善高温对稻米品质的影响。
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3. Characterization of dual enzyme resulted from bicistronic expression of two β-glucanases in porcine cells
ZHANG Xian-wei, LI Zi-cong, MENG Fan-ming, WANG De-hua, LIU De-wu, HE Xiao-yan, SUN Yue, BAI Yin-shan, WU Zhen-fang
Journal of Integrative Agriculture    2015, 14 (4): 732-740.   DOI: 10.1016/S2095-3119(14)60788-7
摘要2242)      PDF    收藏
Many animal feed grains contain high β-glucan in the cell wall. Pigs do not secret β-glucanase to degrade the β-glucan in their feed. The indigestible β-glucan not only blocks the release of nutrients from the grain cell wall, but also increases the digesta viscosity in the gastrointestinal tract of pigs. Therefore, dietary β-glucan significantly inhibits nutrient digestion and absorption in pigs. Transgenic expression of β-glucanase in the digestive tract of pigs may offer a solution to solve this problem. In the current study, four arti?cial codon-optimized β-glucanases genes was prepared and expressed in porcine cells. Only pBgA and pEgx showed high activity in transfected pig kidney cells. To improve the pH range and pH stability of β-glucanase, the two β-glucanases, pBgA and pEgx, were co-expressed in pig kidney cells and salivary gland cells by Linker A3 or 2A peptide. The resulting dual enzymes of pBgA3pEg and pBg2ApEg showed significantly enlarged pH range and significantly increased pH stability, as compared to parental enzymes. These results provide useful data for future study on increasing the feed digestibility of pigs by transgenic expression of β-glucanase in their salivary glands.
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