Copper nanoparticle is a new material widely used in biological medicine, animal husbandry and industrial areas, but its potential toxicity to human health and environment remains unclear. In order to study the hepatotoxic mechanisms of nanoparticles copper, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI/TOF MS) of proteomics technology were used to isolate and identify the differentially expressed proteins from liver, which associated with hepatotoxicity induced by copper nanoparticle in rats. In this study, we have screened 15 kinds of proteins related with hepatotoxicity, of which spot8212 was identified as Malate dehydrogenase (Mdh1). The mRNA expression trend of Mdh1 was consistent with the result of 2-DE by RT-PCR validation. Bioinformatics analysis showed that Mdh1 was stable and no signal peptides, subcellular location was in endoplasmic reticulum; it contained many functional sites such as malate dehydrogenase activity signal sites 155LTRLDHNRAKSQI167; α helixes and random coils were the two main elements. Homologous analysis demonstrated high homologous of Mdh1 in rats with mouse and human, and the phylogenetic tree of Mdh1 was constructed. The result indicated that copper nanoparticle could regulate up the Mdh1 protein expression so as to compensate the energy deficit. Energy metabolic disturbance may be a pathway for copper nanoparticle particles to exert the hepatotoxic effects in rats.

The clinical symptoms of a disorder which is known locally as “wool-eating ailment” in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emaciation, and wool-eating. The mineral composition of forage, and samples of blood, liver and wool from Guizhou semi-fine wool sheep in affected ranches were compared with those from the unaffected areas. The mean concentration of iron in forage from affected pasture was significantly higher than that from the unaffected pasture. The mean concentration of sulfur in forage from the affected and unaffected pasture was similar and within normal ranges. The mean concentration of sulfur in blood, liver and wool from the affected Guizhou semi-fine wool sheep was significantly lower than that from the unaffected sheep while the content of iron in blood, liver and wool from the affected sheep was significantly higher than that from the unaffected sheep. Oral administration of calcium sulfate prevented and cured the disease. We concluded that the disease of Guizhou semi-fine wool sheep in the Weining County is due to S deficiency caused by the high Fe in forages.