本研究比较了分别摄取了dsHvβ′COPI（外壳蛋白复合物I，β′亚基）和dsGFP的茄二十八星瓢虫（Henosepilachna viginitoctopunctata）转录组，以期了解外源dsRNA在茄二十八星瓢虫体内引起的脱靶效应。RNA-Seq结果显示，与dsGFP对照组相比，dsHvβ′COPI处理组中分别有63个上调和44个下调的差异表达基因（DEG）。通过荧光定量PCR（RT-qPCR）分析验证选取的DEG的差异表达，证实了转录组分析结果是可靠的。进一步的分析表明，在茄二十八星瓢虫转录组中没有与Hvβ'COPI同源的基因。此外，在转录组中未发现与dsHvβ'COPI连续匹配>11 bp的基因。我们选择了6个可能参与茄二十八星瓢虫中Hvβ'COPI所参与的代谢路径基因（Hvcitron、Hvhelicase、Hvtransportsase、Hvserine、Hvdynein和HvE3 ubiquitin）来检测dsHvβ'COPI可能引起的脱靶效应。利用RNAi评估这6个脱靶基因的沉默效率。然而，RNAi结果表明这6个基因的表达量虽下调，但对茄二十八星瓢虫无明显的致死作用。此外，本研究的结果将有助于将来RNAi介导的害虫防治的风险分析。

The present study reports the sublethal effects of the entomopathogenic fungus, Aschersonia aleyrodis (Webber) on Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae).  A fungal suspension of A. aleyrodis isolate Aa005 containing 1×10⁷ conidia mL^–1 was sprayed against B. tabaci on eggplant leaves under greenhouse conditions.  The effects of fungal application on survival as well as life table parameters of the whitefly were observed at different post inoculation periods.  The results indicated that A. aleyrodis can significantly affect the survival of 1st, 2nd, and 3rd nymphal instars of B. tabaci.  Developmental periods of different instar nymphs were not affected by fungal application.  A. aleyrodis spores persisted well and significantly affected the survivorship of young instar nymphs of B. tabaci at different post incubation periods.  Life table results suggested that A. aleyrodis has no impact on general fecundity and longevity of B. tabaci.  When the pathogen was exposed to the open environment and maintained for a relatively longer period, a reduction in the reproductive rate and intrinsic rate of increase was caused by the fungal spores, though the sublethal effects were not as good as the control treatment.  The results suggest that the ability of spores to suppress an increase in whitefly population persists well after incubation of spores to the external environment (up to 9 days).  

The sweetpotato whitefly Bemisia tabaci (Homoptera: Aleyrodidae) is a destructive pest of agriculture and horticulture worldwide. Recent phylogenetic analysis using mitochondrial cytochrome oxidase 1 sequences indicates that this whitefly is a species complex including at least 24 morphologically indistinguishable but genetically distinct cryptic species. In this study, the inter-species crosses of Middle East-Asia Minor 1 (MEAM1), Mediterranean (MED) and Asia II 7 cryptic species, which were referred to as B, Q and Cv biotypes before, were conducted in two different devices, leaf cages (7 cm3) and cylinder cages (280 cm3), and at three temperatures of 22, 30 and 38°C. Results indicated that no female progeny were produced in the reciprocal cross between MEAM1×Asia II 7, between MED×Asia II 7 cryptic species neither in leaf cage nor in cylinder cages, while 0.81 and 1.37% of females in the offspring were recorded in the reciprocal cross between MEAM1×MED in leaf cage experiments. Approximately 0.95-0.98% female progeny were recorded in the reciprocal cross between MEAM1×MED at 30°C, 0.77% female progeny were recorded in the single cross direction between MEAM1 × MED at 22°C, and no female progeny were found in their reciprocal cross at 38°C in leaf cage. Our findings indicated that neither space dimension nor temperature have a significant effect on the hybridization of different B. tabaci cryptic species.

The blue gum chalcid, Leptocybe invasa Fisher & La Salle, invaded China in 2007 and has subsequently caused substantial damage to eucalyptus trees. In the current paper, we investigated the susceptibility of 10 Eucalyptus spp. and Eucahetus dunnii to L. invasa in the field, determined the density of galls as well as the gall volume on these tree species, and monitored the population dynamics of wasps in Hainan and Guangdong provinces of China. The order of susceptibility to L. invasa was Eucalyptus urophylla×Eucalyptus camaldulensis>E. urophylla (coppices)>Eucalyptus exserta> Eucalyptus grandis×E. urophylla in Hainan, and Eucalyptus propinqua>Eucalyptus saligna>E. exserta>Eucalyptus microcorys>Eucahetus dunnii>E. camaldulensis>Eucalyptus tereticornis>Eucalyptus robust in Guangdong, China. Although L. invasa generally damages the midribs and petioles of young leaves and the tender bark of twigs of eucalyptus, galls were not observed on leaves of E. microcorys, E. camaldulensis, or E. dunnii. Gall volume significantly differed among the tree species, and gall volume and wasp number were positively correlated. In Dongfang, Hainan Province, the overwintering period of L. invasa emergencing through the year was from the end of December to March of the next year, and the number of population was the greatest on E. urophylla×E. camaldulensis, and the smallest on E. grandis×E. urophylla. In Guangzhou, Guangdong Province, L. invasa hardly emerged in winter from December to June of the next year, and the population was the greatest on E. propinqua, and the smallest on E. microcorys.