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1. StOFP20通过和TONNEAU1 Recruiting Motif 蛋白互作来调控块茎形状
AI Ju, WANG Ye, YAN Ya-wen, LI Chen-xiao, LUO Wei, MA Ling, SHANG Yi, GAO Dong-li
Journal of Integrative Agriculture    2023, 22 (3): 752-761.   DOI: 10.1016/j.jia.2022.08.069
摘要374)      PDF    收藏

OFP蛋白是植物特有的蛋白家族,参与植物生长发育的多个过程。在番茄中,OFP20通过和TRM蛋白互作来调控果实形状。本研究发现敲除StOFP20使二倍体马铃薯C151的薯形由圆形变为椭圆形,从而证实了StOFP20调控薯形的功能。StOFP20的表达在块茎起始期表达最高,随着薯块发育,其表达逐渐降低。为了揭示StOFP20的调控机制,我们在马铃薯中找到了23个TRM基因,其中23个基因在C151中成功扩增。酵母双杂交和荧光素酶互补实验结果表明StOFP203个TRM蛋白互作。缺失StOFP20中的OVATE结构域使蛋白间不能互作,缺失TRM蛋白中的M8结构域则对蛋白互作产生不同的影响。总之,StOFP20和SlOFP20均能和TRM蛋白互作,但是互作蛋白并不完全相同,暗示着它们的调控机制也可能不尽相同。

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2. YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development
WANG Zhong-wei, ZHANG Tian-quan, XING Ya-di, ZENG Xiao-qin, WANG Ling, LIU Zhong-xian, SHI Jun-qiong, ZHU Xiao-yan, MA Ling, LI Yun-feng, LING Ying-hua, SANG Xian-chun, HE Guang-hua
Journal of Integrative Agriculture    2016, 15 (05): 944-953.   DOI: 10.1016/S2095-3119(15)61310-7
摘要1656)      PDF    收藏
    The nuclear-encoded light-harvesting chlorophyll a/b-binding proteins (LHCPs) are specifically translocated from the stroma into the thylakoid membrane through the chloroplast signal recognition particle (cpSRP) pathway. The cpSRP is composed of a cpSRP43 protein and a cpSRP54 protein, and it forms a soluble transit complex with LHCP in the chloroplast stroma. Here, we identified the YGL9 gene that is predicted to encode the probable rice cpSRP43 protein from a rice yellow-green leaf mutant. A phylogenetic tree showed that an important conserved protein family, cpSRP43, is present in almost all green photosynthetic organisms such as higher plants and green algae. Sequence analysis showed that YGL9 comprises a chloroplast transit peptide, three chromodomains and four ankyrin repeats, and the chromodomains and ankyrin repeats are probably involved in protein-protein interactions. Subcellular localization showed that YGL9 is localized in the chloroplast. Expression pattern analysis indicated that YGL9 is mainly expressed in green leaf sheaths and leaves. Quantitative real-time PCR analysis showed that the expression levels of genes associated with pigment metabolism, chloroplast development and photosynthesis were distinctly affected in the ygl9 mutant. These results indicated that YGL9 is possibly involved in pigment metabolism, chloroplast development and photosynthesis in rice.
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3. Biotic Homogenization Caused by the Invasion of Solidago canadensis in China
CHEN Guo-qi, ZHANG Chao-bin, MA Ling, QIANG Sheng, John A Silander , Li Li Qi
Journal of Integrative Agriculture    2013, 12 (5): 835-845.   DOI: 10.1016/S2095-3119(13)60302-0
摘要1777)      PDF    收藏
Although studies argue that invasive species can cause biotic differentiation, some cases show that biological invasions actually decrease biodiversity through biotic homogenization. The concept of biotic homogenization through the invasion of a certain serious invasive plant species merit more studies. Hence, we used field surveys to quantitatively compare invasive populations of Solidago canadensis (SC) in China with the control sites (adjacent sites to SC present sites yet without the species) and SC native populations in the USA. We found that plant communities in SC invaded habitats shared similarities with those in SC native ranges. Bray-Curtis similarity clearly showed that the composition of plant communities in SC invaded habitats were similar to those in SC native ranges. Both in the native and introduced range, plant communities with SC present were characterized by SC being dominant, significantly lower species richness, α-diversity and β-diversity, as well as a decrease in the correlation coefficient between geographic distance and floristic similarities. SC favors fertile and moist loam habitat, while it dominated in various habitats in China, where more than 20 different dominants should have occurred. In conclusion, serious invasive species can quickly remodel and homogenize diverse communities by dominating them.
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