期刊
  出版年
  关键词
结果中检索 Open Search
Please wait a minute...
选择: 显示/隐藏图片
1. The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep
DING Yi, ZHOU Shi-wei, DING Qiang, CAI Bei, ZHAO Xiao-e, ZHONG Shu, JIN Miao-han, WANG Xiao-long, MA Bao-hua, CHEN Yu-lin
Journal of Integrative Agriculture    2020, 19 (4): 1065-1073.   DOI: 10.1016/S2095-3119(19)62853-4
摘要230)      PDF    收藏
The CRISPR/Cas9 system has been extensively used to engineer genetic loci for the generation of knockouts, insertions, and point mutations in animal models.  However, many mutations that have been reported in animals are small insertions or deletions.  This study used the CRISPR/Cas9 system to induce large DNA fragment deletions in MSTN via three guide RNAs in sheep.  This successfully achieved the precise gene editing of the ovine MSTN gene by injecting both Cas9 mRNA and sgRNAs into embryos at the one-cell stage.  Of 10 edited animals, 3 animals (30%) exhibited large genomic fragment deletions (~5 kb).  Furthermore, the body weights of these 3 animals were significantly different (P0<0.0001, P15=0.001, P30=0.005, P60=0.027) between lambs with large deletions and wildtype lambs.  In addition, the edited lambs were also significantly different (P0<0.0001, P15<0.0001, P30=0.002, P60=0.011) compared with wildtype.  These results suggest that the generated MSTN knockout sheep is a reliable and effective animal model for further study.  Furthermore, this method is time- and labor-saving, and efficient for the creation of animal models for agriculture, biology, and medicine.
 
参考文献 | 相关文章 | 多维度评价
2. Expression patterns of OCT4, NANOG, and SOX2 in goat preimplantation embryos from in vivo and in vitro
YU Xiao-li, ZHAO Xiao-e, WANG Hua-yan, MA Bao-hua
Journal of Integrative Agriculture    2015, 14 (7): 1398-1406.   DOI: 10.1016/S2095-3119(14)60923-0
摘要2255)      PDF    收藏
The transcription factors, including OCT4, NANOG, and SOX2, played crucial roles in the maintenance of self-renewal and pluripotency in embryonic stem cells (ESCs). They expressed in preimplantation mammalian development with spatio- temporal pattern and took part in regulation of development. However, their expression and roles in goat had not been reported. In the present study, the expression of OCT4, NANOG, and SOX2 in goat preimplantation embryos both in vivo and in vitro were detected by real-time RCR and immunofluorescence. For in vivo fertilized embryos, the transcripts of OCT4, NANOG, and SOX2 could be detected from oocytes to blastocyst stage, their expression in morula and blastocyst stages was much higher than other stage. OCT4 protein was detected from oocyte to blastocyst, but the fluorescence was more located-intensive with nuclei from 8-cell stage, its expression present in both inner cell mass (ICM) and trophoblast cells (TE) at blastocyse stage. NANOG protein was similar to OCT4, the signaling of fluorescence completely focused on cell nuclei, while the SOX2 firstly showed nuclei location in morula. Comparing to in vivo fertilized embryo, the mRNA of these three transcription factors could be detected at 8-cell stage in parthenogenetic embryos (in vitro). Thereafter, the expressional level rose gradually along with embryo development. The locations of OCT4 and NANOG proteins were similar to in vivo fertilized embryos, and they located in cell nuclei from morula to blastocyst stage, while SOX2 protein firstly could be detected in cell nuclei at 8-cell stage. These differences suggested that OCT4, NANOG, and SOX2 played different function in regulating development of goat preimplantation embryos. These results may provide a novel insight to goat embryo development and be useful for goat ESCs isolation.
参考文献 | 相关文章 | 多维度评价
3. Influence of High Temperature Stress on Net Photosynthesis, Dry Matter Partitioning and Rice Grain Yield at Flowering and Grain Filling Stages
Lü Guo-hua, WU Yong-feng, BAI Wen-bo, MA Bao, WANG Chun-yan , SONG Ji-qing
Journal of Integrative Agriculture    2013, 12 (4): 603-609.   DOI: 10.1016/S2095-3119(13)60278-6
摘要1828)      PDF    收藏
Climate change is recognized to increase the frequency and severity of extreme temperature events. At flowering and grain filling stages, risk of high temperature stress (HTS) on rice might increase, and lead to declining grain yields. A regulated cabinet experiment was carried out to investigate effects of high temperature stress on rice growth at flowering and grainfilling stages. Results showed that no obvious decrease pattern in net photosynthesis appeared along with the temperature rising, but the dry matter allocation in leaf, leaf sheath, culm, and panicle all changed. Dry weight of panicle decreased, and ratio of straw to total above ground crop dry weight increased 6-34% from CK, which might have great effects on carbon cycling and green house gas emission. Grain yield decreased significantly across all treatments on average from 15 to 73%. Occurrence of HTS at flowering stage showed more serious influence on grain yield than at grain filling stage. High temperature stress showed negative effects on harvest index. It might be helpful to provide valuable information for crop simulation models to capture the effects of high temperature stress on rice, and evaluate the high temperature risk.
参考文献 | 相关文章 | 多维度评价
4. 绵羊RXFP2基因不完全敲除不影响角的发育,但会导致雄性单侧隐睾
GAO Yawei, XI Siyuan, CAI Bei, WU Tingjie, WANG Qian, Peter KALDS, HUANG Shuhong, WANG Yuhui, HAN Saizheng, PAN Menghao, YANG Chong, KOU Qifang, MA Baohua, WANG Xiaolong, ZHOU Shiwei and CHEN Yulin
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2023.11.045
录用日期: 2023-11-29