Maximum germination distance (MGD) is an important component of Striga resistance in sorghum.  The objective of this study was to determine gene action influencing MGD of Striga hermonthica and Striga asiatica among selected sorghum lines treated with a biocontrol agent, Fusarium oxysporum f. sp. strigae (FOS) for effective breeding with Striga resistance, and FOS compatibility.  Twelve sorghum genotypes were selected based on their Striga resistance, FOS compatibility, and superior agronomic performance.  Selected genotypes were crossed using a bi-parental mating design to generate six families for genetic analysis.  Agar-gel assays were used to determine low haustorium initiation factor (LHF) using the 12 parental lines, their F₁ progenies, backcross derivatives, and F2 segregants in two sets.  One set had S. hermonthica seed and the other one had S. asiatica seed.  Both were treated with and without FOS.  Genotypes were evaluated using a split-plot design with three replications and MGD data were recorded followed by generation mean analysis.  FOS reduced MGD by 1 cm under both S. hermonthica and S. asiatica infestations.  Additive, dominance, and epistatic gene actions were involved in the control of MGD of the two Striga species in the evaluated populations.  On average, the relative contribution of additive, additive×additive and dominance×dominance genetic effects on the MGD of S. hermonthica and S. asiatica, with FOS, were 20, 33, and 36%; and 21, 32, and 35%, respectively.  Breeding methods exploiting these genetic effects may provide enhanced response to selection for Striga resistance and FOS compatibility in integrated Striga management (ISM) programmes.