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1. 分次施磷通过促进小穗发育和结实缓解低温对小麦的影响
XU Hui, HOU Kuo-yang, FANG Hao, LIU Qian-qian, WU Qiu, LIN Fei-fei, DENG Rui, ZHANG Lin-jie, CHEN Xiang, LI Jin-cai
Journal of Integrative Agriculture    2023, 22 (12): 3667-3680.   DOI: 10.1016/j.jia.2023.09.013
摘要159)      PDF    收藏

随着气候变化的加剧,极端低温事件频发重发。在我国黄淮海麦区,拔节孕穗期(药隔期)发生的倒春寒对冬小麦造成了显著的产量损失。为探索一种经济、可行、高效的小麦低温抗性高产栽培技术本研究以烟农19为试验材料,探讨了次施磷对药隔期低温处理下小麦抗氧化特性和碳氮代谢生理的影响。处理包括传统施磷和分次施磷,然后在药隔期进行-4℃低温处理和自然温度处理。结果表明,与传统施磷相比,分次施磷提高了叶片的净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr),降低了叶片胞间二氧化碳浓度(Ci)。同时,分次施磷处理提高了小麦幼穗碳氮代谢相关酶活性,促进了可溶性糖(SSC)、蔗糖(SUC)、可溶性蛋白(SP)和脯氨酸(Pro)在幼穗中的积累,降低了丙二醛(MDA)的毒性。由于分次施磷增加了生殖发育所需的有机营养,小麦幼穗对低温胁迫的抗性增强,每穗不育小穗数(SSN)传统施磷降低11.8%,小穗结实率(SSR)和最终产量比传统施磷分别提高6.0%8.4%。随着低温处理结束时间的延长分次施磷改善作用更加明显。

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2. Morphological and Hormonal Identification of Porcine Atretic Follicles and Relationship Analysis of Hormone Receptor Levels During Granulosa Cell Apoptosis In vivo
YU De-bing, YU Min-li, LIN Fei, JIANG Bao-chun, YANG Li-na, WANG Si-yu, ZHAO Ying , WNAG Zheng-chao
Journal of Integrative Agriculture    2014, 13 (5): 1058-1064.   DOI: 10.1016/S2095-3119(13)60448-7
摘要1886)      PDF    收藏
Recent reports have demonstrated that follicular atresia is initiated or caused by granulosa cell apoptosis followed by theca cell degeneration in mammalian ovaries, but the mechanism of follicular atresia is still to be elucidated. Therefore, our present study was designed to examine our hypothesis that the changes of follicular microenvironment induce the granulosa cell apoptosis during pocrine follicular atresia in vivo. We firstly isolated intact porcine antral follicles and identified them into three groups, healthy follicles (HF), early atretic follicles (EAF) and progressed atretic follicles (PAF) through morphology and histology. To further confirm their status, we detected hormone levels in follicular fluids and the expression level of apoptosis gene Bax in granulosa cells. The rate of progesterone (P) and estradiol (E2) was increased with the expression of Bax, indicating hormone can be used as a marker of granulosa cell apoptosis or follicular atresia. Finally, we analyzed the expression level of hormone receptor genes in granulosa cells and their relationship with follicular atresia. In PAF, the expression of Progesterone receptor (PGR) was increased significantly while estradiol receptor (ER) had no notable changes, which suggesting the increased-PGR accelerated the effect of P-stimulated granulosa cell apoptosis. The dramatic increasing of androgen receptor (AR) expression in PAF and the obvious increase of tumor necrosis factor-α receptor (TNFR) in EAF indicated that there are different pathways regulating granulosa cell apoptosis during follicular atresia. Together, our results suggested that different pathways of granulosa cell apoptosis was induced by changing the follicular microenvironment during follicular atresia.
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