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1. Heredity and gene mapping of a novel white stripe leaf mutant in wheat
LI Hui-juan, JIAO Zhi-xin, NI Yong-jing, JIANG Yu-mei, LI Jun-chang, PAN Chao, ZHANG Jing, SUN Yu-long, AN Jun-hang, LIU Hong-jie, LI Qiao-yun, NIU Ji-shan
Journal of Integrative Agriculture    2021, 20 (7): 1743-1752.   DOI: 10.1016/S2095-3119(20)63345-7
摘要133)      PDF    收藏

斑点叶(spl)突变体属于一种植物类病斑突变体。我们通过甲基磺酸乙酯(EMS)诱变小麦栽培品种国麦301,获得了一些类病斑突变体,其中一种因其叶片上有白色条纹而被命名为白色条纹叶(wsl)突变体。本文报告这个新的小麦突变体wsl的遗传和基因定位。在整个生育期,突变体wsl的叶片上均有白色条纹出现。随着植株的生长,白色条纹加重,坏死斑面积增大。突变体wsl在拔节前长势较弱,拔节过后长势逐渐恢复。突变体wsl旗叶的长和宽、单株有效穗数和千粒重均显著低于其野生型亲本国麦301。遗传分析表明,wsl的白色条纹性状受1对隐性核基因控制,命名为wsl。利用两个F2群体(wsl×中国春和wsl×济麦22),通过SSR分子标记连锁分析,将wsl基因定位在小麦6BS染色体臂上。在wsl×中国春杂交分离群体中,筛选到4个与wsl基因连锁的标记,Xgpw1079-Xwmc104-Xgwm508-wsl-Xgpw7651,遗传距离分别为7.1, 5.2, 8.7和4.4 cM。在wsl×济麦22杂交分离群体中,筛选到3个与wsl基因连锁的标记,Xgwm508-Xwmc494-Xgwm518-wsl,遗传距离分别为3.5, 1.6和8.2 cM。参考中国春基因组序列,wsl位于6BS染色体88 Mb (Xgwm518)~ 179 Mb (Xgpw7651)之间的91 Mb物理区间内。突变体wsl是研究小麦叶片发育分子机制的重要新种质


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2. Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP
ZHU Xin-xin, NI Yong-jing, HE Rui-shi, JIANG Yu-mei, LI Qiao-yun, NIU Ji-shan
Journal of Integrative Agriculture    2019, 18 (3): 532-538.   DOI: 10.1016/S2095-3119(18)61935-5
摘要194)      PDF(pc) (333KB)(202)    收藏
We identified a wheat (Triticum aestivum L.) multi-pistil mutant from an F2 breeding population in 2012, named 12TP (three pistils in one floret).  Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait.  Using homozygous normal and multi-pistil lines (near-isogenic lines; NILs) derived from the original mutant 12TP, a simple sequence repeat (SSR) marker assay located the 12TP locus on chromosome arm 2DL.  Four SSR markers were linked to 12TP and their order was Xcfd233→Xcfd62-12TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively.  The average genetic expressivity of the trait ‘three pistils in one floret’ was more than 98% in seven homozygous 12TP lines; however, the average genetic expressivity in heterozygous F1 plants was about 49%.  Thus, the 12TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants.  Mutant 12TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat. 
 
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3. Determination of Optimum Growing Degree-Days (GDD) Range Before Winter for Wheat Cultivars with Different Growth Characteristics in North China Plain
LI Qiao-yun, LIU Wan-dai, LI Lei, NIU Hong-bin, MA Ying
Journal of Integrative Agriculture    2012, 12 (3): 405-415.   DOI: 10.1016/S1671-2927(00)8558
摘要1888)      PDF    收藏
To provide base for adjusting the sowing date, achieving the yield potential of wheat cultivars with different growth characteristics, and improving the utilization rate of natural resource in the North China Plain (NCP), a 4-yr field experiment of growing degree-days (GDD) before winter (realized through different sowing dates) with three wheat (Triticum aestivum L.) cultivars of each type of semi-winterness and weak springness was carried out at 20 test experimental sites (32°4´N- 36°1´N) of Henan Province in the NCP. The results showed that: (i) yield of semi-winterness wheat was significantly higher than weak springness wheat (**P<0.01); (ii) there was a quadratic regression between the yield and GDD before winter. According to the regression equation, the optimum GDD range with high yield of semi-winterness and weak springness wheats was 750-770 and 570-590°C d, respectively; (iii) under the optimum GDD condition, the foliar age on the main stem of semi-winterness and weak springness wheats was 7.67-7.91 and 6.36-6.86 leaves, respectively, calculated by the linear regression equation between foliar age and GDD before winter; (iv) both semi-winterness and weak springness wheats were in the double ridge stage of spike differentiation under the condition of the optimum GDD range, and at this time, the foliar age on the main stem of semi-winterness and weak springness wheats was about 7.80 and 6.07 leaves, respectively, which was consistent with the results calculated by the liner regression equation. Therefore, we could consider that the sowing date is appropriate if the foliar age is about 7.8 and 6.3 leaves for semi-winterness and weak springness wheats, respectively. According to the results of this study, choosing semi-winterness wheat and planting 7- 10 d earlier would improve yield and natural resource utilization in NCP.
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