The aim of this study was to investigate the impact of heat stress on physiological features, together with endogenous hormones and the transcription level of related genes, to estimate the heat resistance ability and stress injury mechanism of different dwarfig apple rootstocks. Among the six rootstocks, the rootstocks of native Shao series (SH series) showed better heat stress resistance than those of Budagovski 9 (B9), Cornell-Geneva 24 (CG24), and Malling 26 (M26) from abroad. Among SH series rootstocks, SH1 and SH6 showed higher heat stress resistance than SH40. M26 demonstrated the lowest adaption ability to heat stress, showing higher leaf conductivity and lower liquid water content (LWC) with the increase in temperature. Heat stress also resulted in the suppression of photosynthesis, which showed no signifiant restoration after 7-day recovery. It should be noted that although a higher temperature led to a lower LWC and photosynthetic effiiency (P_n ) of CG24, there was no signifiant increase in leaf conductivity, and 7 days after the treatment, the P_n of CG24 recovered. The extremely high temperature tolerance of SH series rootstocks could be related to the greater osmotic adjustment (OA), which was reflcted by smaller reductions in leaf relative water content (RWC) and higher turgor potentials and leaf gas exchange compared with the other rootstocks. Determination of hormones indicated multivariate regulation, and it is presumed that a relatively stable expression levels of functional genes under high-temperature stress is necessary for heat stress resistance of rootstocks