西番莲（Passiflora edulis Sims）也称百香果，是西番莲科西番莲属的藤本植物。其全株提取的黄酮类和萜类对人体有良好的抗焦虑和抗炎作用，西番莲除了鲜食之外还可药用。在这项工作中，我们利用RNA-Seq分析了紫果品种的四个组织的转录表达，注释了大量基因功能。西番莲叶片中的黄酮类和萜类化合物主要是木犀草素、芹菜素、环状三萜皂苷和其他活性物质的衍生物。利用基于同源BLAST和系统发育分析，筛选了转录组数据中可能参与黄酮类和萜类合成途径的一系列候选单基因。结果显示，西番莲的三萜类化合物的生物合成来自甲羟戊酸（MVA）和2-C-甲基-D-赤藓糖醇-4-磷酸/1-脱氧-D-木糖醇-5-磷酸（MEP/DOXP）途径，这与其他果树通过MVA途径为主的生物合成不同。大多数候选基因在叶和/或花中高表达。8个关键基因的定量实时PCR（qRT-PCR）验证，证实了RNA-Seq数据的可靠性，并获得了其在西番莲属八个种（species）和其中一个种（栽培种）的四个组织中的表达规律。这些工作对分析关键基因在西番莲黄酮类化合物和萜类化合物的生物合成提供基础。

In order to solve the problem of how to collect and manage large amount of location information and improve the accuracy of location service, interactive information supporting system based on ontology and location service is presented in this paper. It can transmit special information about users’ location to front-end instantaneously, so it is very convenient for their daily life. It makes full use of the available wireless communication, which is easy to arrange and convenient for users.

Various fishery information systems have been developed in different times and on different platforms. Web service application composition is crucial in the sharing and integration of fishery data and information. In the present paper, a heuristic web service composition method based on fishery ontology is presented, and the proposed web services are described. Ontology reasoning capability was applied to generate a service composition graph. The heuristic function was introduced to reduce the searching space. The experimental results show that the algorithm used considers the services semantic similarity and adjusts web service composition plan by dynamically relying on the empirical data.