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1. 过表达油菜SBPase和cyFBPase基因显著提高了烟草的生长及生物量
LI Yan-yan, GUO Li-na, LIANG Cheng-zhen, MENG Zhi-gang, Syed Tahira, GUO San-dui, ZHANG Rui
Journal of Integrative Agriculture    2022, 21 (1): 49-59.   DOI: 10.1016/S2095-3119(20)63438-4
摘要169)      PDF    收藏

在植物中,胞质果糖-1,6-二磷酸酶(cyFBPase)和景天庚酮糖-1,7-二磷酸酶(SBPase)酶活性的提高与植物增产密切相关。在本研究中,通过在烟草(Nicotiana tabacum)中过表达油菜cDNA,cyFBPaseSBPase基因表达水平显著提高。在转双基因cyFBPase/SBPase (TpFS)植株中, cyFBPase 和 SBPase酶活分别是野生型的1.77和1.45倍,在转cyFBPase单基因(TpF)和SBPase单基因 (TpS)植株中,cyFBPase 和 SBPase酶活分别是野生型的1.55和1.12倍1.23和1.36倍。TpF, TpS 和 TpFS转基因植株的光合效率分别比野生型提高了4%20%和25%。SBPase和cyFBPase在转基因烟草中相互正向调控,协同增效。 此外,三种转基因植株的蔗糖含量均高于WT植株。 淀粉含量在TpFS和TpS植株中分别提高了53%和37%,但在TpF植株中略有下降。 此外,含有SBPase/或cyFBPase基因的转基因烟草植株生长加快,生物量提高 TpFS、TpS和TpF植株中,干重、株高、茎粗、叶大小、花数和果荚重均比WT植株大幅增高。 因此,共表达SBPasecyFBPase可能为作物高产开辟新的途径。

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2. Effects of Cultivar on Phenolic Content and Antioxidant Activity of Naked Oat in China
TONG Li-tao, LIU Li-ya, ZHONG Kui, WANG Yan, GUO Li-na , ZHOU Su-mei
Journal of Integrative Agriculture    2014, 13 (8): 1809-1816.   DOI: 10.1016/S2095-3119(13)60626-7
摘要1165)      PDF    收藏
To evaluate the effects of naked oat cultivars on their phenolic contents and antioxidant activities, the oat seeds from 21 different cultivars in China were collected, and their major nutritional components, compositions of polyphenols, and antioxidant activities were measured. As the results, oat cultivars affected its phenolic composition and antioxidant activity significantly. Vanillin, chlorogenic acid, ferulic acid, avenanthramide C (Bc), avenanthramide A (Bp), and avenanthramide B (Bf) in oat polyphenols extracts were detected in all cultivars of oat, while caffeic acid, p-coumaric acid and rutin were partly detected. All oat cultivars showed significant free radical scavenging activity, although their activities were lower than that of vitamin C (VC). Different oat varieties showed different antioxidant activities, among which Yanke 1 had the best ability to scavenge •OH ((58.78±1.99) μg mL-1), Baiyan 10 showed the strongest DPPH• ((22.00±0.43) μg mL-1) and ABTS•+ ((6.92±0.44) μg mL-1) scavenging activity, and Dingyou 1 showed good scavenging ability of DPPH• ((22.71±0.42) μg mL-1) and •OH ((81.50±1.73) μg mL-1). In addition, the correlation coefficients of contents between the major nutritional components in different cultivars of oat and phenolic compounds indicated that determination of main nutrients could be used as an easy technique to evaluate approximately the contents and compositions of oat polyphenols, which simplifies the selection of good oat cultivar.
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