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1. 柑橘木虱囊泡运输相关基因在黄龙病菌侵染中的作用
Yingzhe Yuan, Tao Peng, Aijun Huang, Jun He, Chenyang Yuan, Tianyuan Liu, Long Yi, Xuejin Cui, Xuefeng Wang, Changyong Zhou
Journal of Integrative Agriculture    2024, 23 (12): 4136-4146.   DOI: 10.1016/j.jia.2024.03.063
摘要82)      PDF    收藏
黄龙病是一种由Candidatus Liberibacter asiaticus (CLas) 引起韧皮部寄生的革兰氏阴细菌,主要由亚洲柑橘木虱以持久增殖的方式传播,是柑橘上最具毁灭性的病害。有研究提出黄龙病菌借助木虱细胞内的囊泡结构来复制和传播。然而,黄龙病菌如何进入木虱细胞以及囊泡运输作用在此过程中起到何种作用目前尚不清楚。本研究中我们监测木虱五龄若虫在不同取食时间后体内CLas滴度的变化,结果表明,若虫体内的CLas滴度随着取食时间的延长而增加。在CLas感染的木虱体内囊泡转运相关基因的表达显著上调,当阻断木虱内吞作用和内体网络后,CLas在木虱整虫和中肠的滴度显著下降。此外通过沉默网格蛋白重链基因(dsCHC1)的表达同样导致了柑橘木虱体内CLas滴度的降低。总而言之,我们的结果表明,CLas感染上调了柑橘木虱囊泡运输相关基因的表达从而促进了依赖内吞作用病原菌的入侵
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2. Molecular mapping of a stripe rust resistance gene in Chinese wheat cultivar Mianmai 41
REN Yong, LI Sheng-rong, WEI Yu-ming, ZHOU Qiang, DU Xiao-ying, HE Yuan-jiang, ZHENG You-liang
Journal of Integrative Agriculture    2015, 14 (2): 295-304.   DOI: 10.1016/S2095-3119(14)60781-4
摘要1808)      PDF    收藏
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2:3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as YrMY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 cM, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and YrCH42 on chromosome 1B. Allelic tests indicated that YrMY41 is likely a new allele at Yr26 locus.
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3. Effects of Temperature on Functional Response of Anagrus nilaparvatae Pang et Wang (Hymenoptera: Mymaridae) on the Eggs of Whitebacked Planthopper, Sogatella furcifera Horváth and Brown Planthopper, Nilaparvata lugens Stål
MA Ming-yong, PENG Zhao-pu, HE Yuan
Journal of Integrative Agriculture    2012, 12 (8): 1313-1320.   DOI: 10.1016/S1671-2927(00)8660
摘要1372)      PDF    收藏
Understanding the temperature affecting parasitic efficiency is critical to succeed in utilizing parasitoid as natural enemy in pest management. Laboratory studies were carried out to determine the effects of temperature on parasitoid preference of female Anagrus nilaparvatae Pang et Wang (Hymenoptera: Mymaridae) to the eggs of whitebacked planthopper (WBPH), Sogatella furcifera Horváth and brown planthopper (BPH), Nilaparvata lugens Stål to build a composite model describing changes in parasitic response along a temperature gradient (18, 22, 26, 30, 34°C). The results showed that attack responses of A. nilaparvatae on WBPH and BPH were the best described by a Type II functional response. The two parameters, attack rates (a) and handling times (Th), of A. nilaparvatae to both eggs were influenced by the temperature. The maximum attack rates to WBPH (1.235) and BPH (1.049) were at 26 and 34°C, respectively, and the shortest handling times to WBPH (0.063) and BPH (0.057) were at 30 and 26°C, respectively. However, the optimal temperature for parasitic efficiency of A. nilaparvatae to WBPH and BPH eggs was both at 26°C, which showed that the present microclimate temperature of the habitat in the paddyfield was beneficial to A. nilaparvatae and indicated that parasitic efficiency of A. nilaparvatae would be impaired by global warming.
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