Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency

doi:10.1016/S2095-3119(14)60860-1

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (5): 888-899.DOI: 10.1016/S2095-3119(14)60860-1

Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency

YU Chao, CHEN Hua-min, TIAN Fang, BI Yong-mei, Rothstein J Steven, Leach E Jan, HE Chen-yang

1、State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1, Canada
3、Department of Bioagricultural Sciences and Pest Management, Colorado State University, Ft. Collins CO 80523-1177, USA

收稿日期:2014-05-12 出版日期:2015-05-01 发布日期:2015-05-13
通讯作者: HE Chen-yang, Tel: +86-10-62894147, E-mail: hechenyang@caas.cn
作者简介:YU Chao, E-mail: yudoxichao@163.com;* These authors contributed equally to this study.
基金资助:
This work was supported by the grants from the National Basic Research Program of China (2011CB100701).

Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency

YU Chao, CHEN Hua-min, TIAN Fang, BI Yong-mei, Rothstein J Steven, Leach E Jan, HE Chen-yang

1、State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph ON N1G 2W1, Canada
3、Department of Bioagricultural Sciences and Pest Management, Colorado State University, Ft. Collins CO 80523-1177, USA

Received:2014-05-12 Online:2015-05-01 Published:2015-05-13
Contact: HE Chen-yang, Tel: +86-10-62894147, E-mail: hechenyang@caas.cn
About author:YU Chao, E-mail: yudoxichao@163.com;* These authors contributed equally to this study.
Supported by:
This work was supported by the grants from the National Basic Research Program of China (2011CB100701).

摘要/Abstract

摘要： Bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of high nitrogen (N) responsive diseases. Rice plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might exist. However, the co-regulatory components in such crosstalk have not been elucidated. Here, we comparatively analyzed the gene expression profiling of rice under Xoo inoculation, low N treatment, or a combination of both stresses, and identified the differentially-expressed genes (DEGs) in overlapping responses. These DEGs were involved in different biological processes, including innate immunity and nitrogen metabolism. The randomly-selected DEGs expression was validated by quantitative real-time PCR assays. Temporal expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were present. These DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.

关键词: rice , differentially-expressed genes (DEGs) , bacterial infection , nitrogen deficiency , co-regulation

Abstract: Bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of high nitrogen (N) responsive diseases. Rice plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might exist. However, the co-regulatory components in such crosstalk have not been elucidated. Here, we comparatively analyzed the gene expression profiling of rice under Xoo inoculation, low N treatment, or a combination of both stresses, and identified the differentially-expressed genes (DEGs) in overlapping responses. These DEGs were involved in different biological processes, including innate immunity and nitrogen metabolism. The randomly-selected DEGs expression was validated by quantitative real-time PCR assays. Temporal expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were present. These DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.

Key words: rice , differentially-expressed genes (DEGs) , bacterial infection , nitrogen deficiency , co-regulation

YU Chao, CHEN Hua-min, TIAN Fang, BI Yong-mei, Rothstein J Steven, Leach E Jan, HE Chen-yang. Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency[J]. Journal of Integrative Agriculture, 2015, 14(5): 888-899.

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Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency

Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency

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