Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (2): 208-216.DOI: 10.1016/S2095-3119(14)60889-3

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Alternatives for large-scale production of cultured beef: A review

 Matilda S M Moritz, Sanne E L Verbruggen, Mark J Post   

  1. 1、Department of Physiology, Maastricht University, Maastricht 6229 ER, The Netherlands
    2、Department of Physics, Chemistry and Biology, Linköping University, Linköping SE-581 83, Sweden
  • 收稿日期:2014-04-03 出版日期:2015-02-01 发布日期:2015-02-11
  • 通讯作者: Mark J Post,Tel: +31-43-3881200, Mobile: +31-6-46705558, Fax: +31-43- 3884166, E-mail: m.post@maastrichtuniversity.nl
  • 作者简介:Matilda S M Moritz, E-mail: matilda.moritz@maastrichtuniversity. nl, matmo608@student.liu.se;

Alternatives for large-scale production of cultured beef: A review

 Matilda S M Moritz, Sanne E L Verbruggen, Mark J Post   

  1. 1、Department of Physiology, Maastricht University, Maastricht 6229 ER, The Netherlands
    2、Department of Physics, Chemistry and Biology, Linköping University, Linköping SE-581 83, Sweden
  • Received:2014-04-03 Online:2015-02-01 Published:2015-02-11
  • Contact: Mark J Post,Tel: +31-43-3881200, Mobile: +31-6-46705558, Fax: +31-43- 3884166, E-mail: m.post@maastrichtuniversity.nl
  • About author:Matilda S M Moritz, E-mail: matilda.moritz@maastrichtuniversity. nl, matmo608@student.liu.se;

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摘要： Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today’s 2-dimensional (2D) standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.

关键词: cultured beef , microcarriers , aggregated cells , packed bed bioreactor , cell culture

Abstract: Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today’s 2-dimensional (2D) standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.

Key words: cultured beef , microcarriers , aggregated cells , packed bed bioreactor , cell culture